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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jul 20, 2023 |
| Title |
The impact of small molecule inhibition of METTL3 on normal haematopoiesis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We performed scRNA-seq on haematoietic stem and progenitor cells of healthy wild-type mice treated with the small-molecule inhibitor of METTL3, STM2457.
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| Overall design |
Bone marrow cells were collected from 4 vehicle-treated and 3 STM2457-treated mice and lineage-negative, cKIT positive Haematopoietic stem and progenitor cells were isolated by fluorescence activated cell sorting. Cells were processed for scRNA-seq using Chromium Single Cell 3สน GEM, Library & Gel Bead Kit v3 reagents and sequenced on the Illumina Novaseq 6000 platform.
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| Contributor(s) |
Sturgess K, Yankova E, Vijayabaskar M, Isobe T, Rak J, Kucinski I, Barile M, Webster N, Eleftheriou M, Hannah R, Gozdecka M, Vassiliou G, Rausch O, Wilson N, Gottgens B, Tzelepis K |
| Citation(s) |
37464070 |
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| Submission date |
Mar 30, 2023 |
| Last update date |
Jul 21, 2023 |
| Contact name |
Bertie Gottgens |
| E-mail(s) |
bg200@cam.ac.uk
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| Organization name |
University of Cambridge
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| Street address |
Jeffrey Cheah Biomedical Centre
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| City |
Cambridge |
| ZIP/Postal code |
CB2 0AW |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA950362 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE228562_GEO_submission_adata.h5ad.gz |
483.3 Mb |
(ftp)(http) |
H5AD |
| GSE228562_RAW.tar |
731.5 Mb |
(http)(custom) |
TAR (of H5) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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