 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Feb 21, 2024 |
| Title |
scRNA-seq analysis of tumor-infiltrating CD45+ cells from sitagliptin-treated mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Dendritic cell (DC)-mediated antigen presentation is essential for the priming and activation of tumor-specific T cells. However, few drugs that specifically manipulate DC functions are available. Here, we observed that DCs initiated a distinct transcriptional program during antigen presentation. We utilized a network-based approach to screen for DC-targeting therapeutics. Sitagliptin, an oral gliptin widely used for type 2 diabetes, was identified as a drug that targets DCs. In mouse models, sitagliptin inhibited tumor growth by enhancing DC-mediated antigen presentation, leading to better T cell activation.
|
| |
|
| Overall design |
MC38-OVA tumor-bearing mice were fed control or sitagliptin diet. CD45+ cells were sorted from tumor tissues. ScRNA-seq was performed.
|
| |
|
| Contributor(s) |
Tang H |
| Citation(s) |
38458637 |
| |
| Submission date |
Mar 24, 2023 |
| Last update date |
May 22, 2024 |
| Contact name |
Haidong Tang |
| E-mail(s) |
tanghaidong06@gmail.com
|
| Organization name |
Tsinghua University
|
| Street address |
Shuangqing Road
|
| City |
Beijing |
| ZIP/Postal code |
100084 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA948366 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE228128_RAW.tar |
128.9 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
