|
| Status |
Public on Jun 26, 2023 |
| Title |
Effect of SERT LOF on Drosophila Kenyon Cell subtypes postsynaptic to serotonin [scRNA-seq] |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
to investigate the transcriptional effects on postsynaptic cells following SSRI-like SERT LOF, we used Drosophila mutants for SERT and compared to controls, isolating and profiling Kenyon Cells of the mushroom body
|
| |
|
| Overall design |
Kenyon cells of SERT LOF or WT groups were isolated (FACS) according to the presence of nls.GFP signal and analyzed using scRNAseq.
|
| Web link |
https://www.nature.com/articles/s41398-023-02521-3
|
| |
|
| Contributor(s) |
Bonanno S, Krantz D |
| Citation(s) |
37355701 |
| |
| Submission date |
Mar 21, 2023 |
| Last update date |
Jun 29, 2023 |
| Contact name |
Shivan Luca Bonanno |
| E-mail(s) |
shivan.bonanno@gmail.com
|
| Phone |
5302203672
|
| Organization name |
UCLA
|
| Department |
Psychiatry
|
| Lab |
Krantz
|
| Street address |
Gonda Rm3335, 695 Charles E. Young Dr.
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL25244 |
Illumina NovaSeq 6000 (Drosophila melanogaster) |
|
| Samples (3) |
| GSM7109953 |
Mef2G4, dSERT4 v dSERT16, day0, scRNAseq |
| GSM7109954 |
Mef2G4, dSERT-TMKO v WT, day0, scRNAseq |
| GSM7109955 |
Mef2G4, dSERT-TMKO v WT and CIT v VEH, day4to6, scRNAseq |
|
| This SubSeries is part of SuperSeries: |
| GSE227935 |
Transcriptional changes in specifc subsets of Drosophila neurons following inhibition of the serotonin transporter |
|
| Relations |
| BioProject |
PRJNA947206 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE227867_RAW.tar |
91.5 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
| GSE227867_SeuratObj1_coarse.rds.gz |
312.7 Mb |
(ftp)(http) |
RDS |
| GSE227867_SeuratObj2_KCs.rds.gz |
197.9 Mb |
(ftp)(http) |
RDS |
| GSE227867_SeuratObj3_usedForDE.rds.gz |
189.5 Mb |
(ftp)(http) |
RDS |
| GSE227867_dgrp2.dm6.subset.pileup.vcf.gz |
7.0 Mb |
(ftp)(http) |
VCF |
| GSE227867_scRNA-seq_DEGs_CIT_v_VEH_Day4.csv.gz |
946.1 Kb |
(ftp)(http) |
CSV |
| GSE227867_scRNA-seq_DEGs_dSERT-TMKO_v_WT_Day0.csv.gz |
1.1 Mb |
(ftp)(http) |
CSV |
| GSE227867_scRNA-seq_DEGs_dSERT-TMKO_v_WT_Day4.csv.gz |
861.0 Kb |
(ftp)(http) |
CSV |
| GSE227867_scRNA-seq_DEGs_dSERT16_v_dSERT4_Day0.csv.gz |
1011.1 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
