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| Status |
Public on Jan 14, 2024 |
| Title |
The effects of WTAP on heart development [WTAPCKO] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The goal of this study is to investigate how WTAP regulates heart development.
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| Overall design |
mRNA profiles in the hearts of Wtapflox/flox and Wtap-CKO mice were generated by deep sequencing using Illumina Novaseq 6000 platform (n=3 for each group).
Total RNA was extracted using Tripure Isolation Reagent (Roche, Mannheim, Germany) from the hearts of Wtapflox/flox and Wtap-CKO mice at 4 days old. Three independent biological replicates for each group were used for RNA-seq. RNA-seq was performed by deep sequencing using an Illumina Novaseq 6000 platform. Paired-end clean reads were aligned to the mouse reference genome(GRCm39) with Hisat2 v2.0.5, and the aligned reads were used to quantify mRNA expression by using featureCounts v1.5.0-p3.
Our study represents the first detailed analysis of heart transcriptomes from Wtapflox/flox and Wtap-CKO mice, generated by RNA-seq technology. The RNA-seq analysis showed that 2348 genes were downregulated and 2764 genes were upregulated in the hearts of Wtap-CKO mice.
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| Contributor(s) |
Chen Z, Li X, Shi L, Zhang Z |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Mar 12, 2023 |
| Last update date |
Jan 15, 2024 |
| Contact name |
Zheng Chen |
| E-mail(s) |
chenzheng@hit.edu.cn
|
| Phone |
+8645186402029
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| Organization name |
Harbin Institute of Technology
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| Street address |
92 Xidazhijie Harbin Institute of Technology
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| City |
Harbin |
| State/province |
Heilongjiang |
| ZIP/Postal code |
150001 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA943502 |
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