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Series GSE227149 Query DataSets for GSE227149
Status Public on Jun 26, 2024
Title The Janus kinase 1 is critical for the postnatal development of the pancreas and cancer progression
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Inflammatory cytokines promote the development of pancreatic ductal adenocarcinoma (PDAC), but the functions of specific intracellular signaling mediators in this process are less well-defined. In this study, we discovered that the Janus kinase 1 (JAK1) is critical for the early postnatal growth of the exocrine pancreas.
Using a ligand-controlled and pancreas-specific knockout in adult mice, we demonstrate that JAK1 deficiency prevents the formation of KRASG12D-induced pancreatic tumors, and we established that JAK1 is essential for the constitutive activation of STAT3 whose activation is a prominent characteristic of PDAC.
We identified C/EBPδ as a biologically relevant downstream target of JAK1 signaling, which is upregulated in human PDAC. Reinstating the expression of C/EBPδ was sufficient to rescue the growth of JAK1-deficient tumorspheres.
Collectively, the findings of this study suggest that JAK1 executes important functions of inflammatory cytokines through C/EBPδ and may serve as a molecular target for PDAC prevention and treatment.
 
Overall design Comparative gene expression profiling analysis of RNA-seq data forsix isogenic pairs of mouse pancreatic cancer cell lines before and after Cre-mediated deletion of JAK1.
 
Contributor(s) Shrestha H, Rädler PD, Wagner K
Citation(s) 38733583
Submission date Mar 11, 2023
Last update date Jun 26, 2024
Contact name Kay-Uwe Wagner
E-mail(s) wagnerk@karmanos.org
Organization name Barbara Ann Karmanos Cancer Institute Wayne State University School of Medicine
Department Department of Oncology
Street address 421 East Canfield, EL01TM
City Detroit
State/province MI
ZIP/Postal code 48201-1976
Country USA
 
Platforms (2)
GPL19057 Illumina NextSeq 500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (12)
GSM7092423 52205 (WT)
GSM7092424 52205-cre (JAK1 KO)
GSM7092425 52419 (WT)
Relations
BioProject PRJNA943409

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE227149_Normalized_Gene_Count.txt.gz 1005.4 Kb (ftp)(http) TXT
GSE227149_Normalized_Log2_Gene_Count.txt.gz 715.7 Kb (ftp)(http) TXT
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