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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 12, 2023 |
| Title |
Saturating mutagenesis of the GADD45A intron 3 enhancer using STARRseq |
| Organisms |
Homo sapiens; synthetic construct |
| Experiment type |
Other
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| Summary |
Assessment of individual nucleotide impact on overall GADD45A enhancer activity, dependency on p53 and/or activation through inegrated stress response (ISR).
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| Overall design |
Massively parallel reporter assay (MPRA) testing every nucleotide substitution or deletion within the GADD45A intron 3 enhancer
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| Contributor(s) |
Sammons MA, Baniulyte G |
| Citation(s) |
36993734 |
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| Submission date |
Mar 03, 2023 |
| Last update date |
Apr 04, 2023 |
| Contact name |
Morgan A Sammons |
| E-mail(s) |
masammons@albany.edu
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| Organization name |
University at Albany - SUNY
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| Department |
Biological Sciences
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| Lab |
Sammons
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| Street address |
1400 Washington Ave, Life Sciences 2075
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| City |
Albany |
| State/province |
NY |
| ZIP/Postal code |
12222 |
| Country |
USA |
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| Platforms (2) |
| GPL30173 |
NextSeq 2000 (Homo sapiens) |
| GPL32628 |
NextSeq 2000 (synthetic construct) |
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| Samples (7)
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| GSM7080408 |
RNA, GADD45A_enh_STARRSeq, HCT116, DMSO |
| GSM7080409 |
RNA, GADD45A_enh_STARRSeq, HCT116, Nutlin-3A |
| GSM7080410 |
RNA, GADD45A_enh_STARRSeq, HCT116, Tunicamycin |
| GSM7080411 |
RNA, GADD45A_enh_STARRSeq, HCT116 p53KO, DMSO |
| GSM7080412 |
RNA, GADD45A_enh_STARRSeq, HCT116 p53KO, Nutlin-3A |
| GSM7080413 |
RNA, GADD45A_enh_STARRSeq, HCT116 p53KO, Tunicamycin |
| GSM7080414 |
DNA, GADD45A_enh_STARRSeq, plasmidPool |
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| Relations |
| BioProject |
PRJNA940815 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE226617_GADD45A_enh_STARRSeq_raw_read_count.csv.gz |
22.7 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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