|
| Status |
Public on Jan 17, 2024 |
| Title |
Efect of Arpp21 deficiency on TCR repertoire diversity |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
To assess the impact of Arpp21 deficiency on the TCR repertoire, we generated Arpp21-deficient mice from which we isolated CD4 T cells and subjected them to NGS sequencing.
|
| |
|
| Overall design |
From the spleens of age-matched mice, 7 of which were WT and 7 of which were Arpp21 KO, 6.8 million CD4 T cells were purified by FACS. Technical details: - Library preparation kit: Takara SMARTer Mouse TCR alpha-beta Profiling Kit - Sample QC method: Qubit and Tapestation - Library QC method: Qubit and Tapestation - Instrument: Illumina MiSeq - Read length: PE300
|
| |
|
| Contributor(s) |
Lyszkiewicz M, Xu M, Heissmeyer V |
| Citation(s) |
38467629 |
| |
| Submission date |
Mar 01, 2023 |
| Last update date |
Mar 20, 2024 |
| Contact name |
Marcin Lyszkiewicz |
| E-mail(s) |
marcin.lyszkiewicz@googlemail.com, marcin.lyszkiewicz@uni-ulm.de
|
| Organization name |
Ulm University Medical Center
|
| Department |
Dpt. of Pediatrics and Adolescent Medicine
|
| Street address |
Eythstr. 24
|
| City |
Ulm |
| ZIP/Postal code |
89073 |
| Country |
Germany |
| |
|
| Platforms (1) |
|
| Samples (14)
|
|
| Relations |
| BioProject |
PRJNA940086 |
Less...
More...