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Series GSE22562 Query DataSets for GSE22562
Status Public on Aug 12, 2010
Title Control of Embryonic Stem Cell State by Mediator and Cohesin (Illumina ChIP-Seq data)
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The key transcription factors that control the embryonic stem cell gene expression program have been identified, but how they function to implement this program is not well understood. While screening for genes essential for maintenance of ES cell state, we identified many components of the Mediator and Cohesin complexes. Mediator and Cohesin were found to physically and functionally connect the enhancers and core promoters of active genes. An ES cell Mediator complex was found to copurify with Cohesin and its loading factor Nipbl, and normal levels of these proteins were essential for expression of the genes they occupy and for maintenance of ES cell state.
Overall design See associated publication.

Sample GSM555166 (GSE22320) was used as a negative control.
Contributor(s) Kagey MH, Bilodeau S, Newman JJ, Orlando DA, Young RA
Citation(s) 20720539
Submission date Jun 24, 2010
Last update date May 15, 2019
Contact name Richard A Young
Phone 617-258-5219
Organization name Whitehead Institute for Biomedical Research
Lab Young Lab
Street address 9 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
Platforms (1)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
Samples (17)
GSM560341 ChIP-Seq for Smc1 in mES cells
GSM560342 ChIP-Seq for Smc1 in mES cells (rep 2)
GSM560343 ChIP-Seq for Smc3 in mES cells (rep 2)
This SubSeries is part of SuperSeries:
GSE22557 Control of Embryonic Stem Cell State by Mediator and Cohesin
SRA SRP002778
BioProject PRJNA129097

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE22562_RAW.tar 599.2 Mb (http)(custom) TAR (of TXT, WIG)
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Processed data provided as supplementary file
Raw data are available in SRA

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