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Series GSE22533 Query DataSets for GSE22533
Status Public on May 14, 2012
Title Breast cancer cells resistant to hormone deprivation maintain an estrogen receptor alpha-dependent, E2F-directed transcriptional program
Organism Homo sapiens
Experiment type Expression profiling by array
Summary A significant fraction of breast cancers exhibit de novo or acquired resistance to estrogen deprivation. To model resistance to aromatase inhibitor (AI) therapy, long-term estrogen-deprived (LTED) derivatives of MCF-7 and HCC-1428 cells were generated through culture for 3 and 7 months under hormone-depleted conditions, respectively. These LTED cells showed sensitivity to the ER downregulator fulvestrant under hormone-depleted conditions, suggesting continued dependence upon ER signaling for hormone-independent growth. To evaluate the role of ER in hormone-independent growth, LTED cells were treated +/- 1 uM fulvestrant x 48 h before RNA was harvested for gene expression analysis.
Overall design MCF-7/LTED and HCC-1428/LTED cells were treated with 10% DCC-FBS with or without the estrogen receptor antagonist drug fulvestrant for 48 hrs prior to RNA harvest for array analysis. Three replicates per condition.
Contributor(s) Miller T, Arteaga C
Citation(s) 22049316
Submission date Jun 23, 2010
Last update date Mar 25, 2019
Contact name Todd W Miller
Organization name Vanderbilt Univ
Department Medicine
Street address 2220 Pierce Ave, 777 PRB
City Nashville
State/province TN
ZIP/Postal code 37174
Country USA
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (12)
GSM559507 MCF-7/LTED, EtOH, rep 1
GSM559508 MCF-7/LTED, EtOH, rep 2
GSM559509 MCF-7/LTED, EtOH, rep 3
This SubSeries is part of SuperSeries:
GSE37955 ERa-dependent E2F transcription can mediate resistance to estrogen deprivation in human breast cancer
BioProject PRJNA128503

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE22533_RAW.tar 60.7 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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