 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on May 14, 2012 |
| Title |
Breast cancer cells resistant to hormone deprivation maintain an estrogen receptor alpha-dependent, E2F-directed transcriptional program |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
A significant fraction of breast cancers exhibit de novo or acquired resistance to estrogen deprivation. To model resistance to aromatase inhibitor (AI) therapy, long-term estrogen-deprived (LTED) derivatives of MCF-7 and HCC-1428 cells were generated through culture for 3 and 7 months under hormone-depleted conditions, respectively. These LTED cells showed sensitivity to the ER downregulator fulvestrant under hormone-depleted conditions, suggesting continued dependence upon ER signaling for hormone-independent growth. To evaluate the role of ER in hormone-independent growth, LTED cells were treated +/- 1 uM fulvestrant x 48 h before RNA was harvested for gene expression analysis.
|
| |
|
| Overall design |
MCF-7/LTED and HCC-1428/LTED cells were treated with 10% DCC-FBS with or without the estrogen receptor antagonist drug fulvestrant for 48 hrs prior to RNA harvest for array analysis. Three replicates per condition.
|
| |
|
| Contributor(s) |
Miller T, Arteaga C |
| Citation(s) |
22049316 |
| |
| Submission date |
Jun 23, 2010 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Todd W Miller |
| E-mail(s) |
todd.miller@vanderbilt.edu
|
| Organization name |
Vanderbilt Univ
|
| Department |
Medicine
|
| Street address |
2220 Pierce Ave, 777 PRB
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37174 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (12)
|
| GSM559510 |
MCF-7/LTED, fulv, rep 1 |
| GSM559511 |
MCF-7/LTED, fulv, rep 2 |
| GSM559512 |
MCF-7/LTED, fulv, rep 3 |
| GSM559513 |
HCC-1428/LTED, EtOH, rep 1 |
| GSM559514 |
HCC-1428/LTED, EtOH, rep 2 |
| GSM559515 |
HCC-1428/LTED, EtOH, rep 3 |
| GSM559516 |
HCC-1428/LTED, fulv, rep 1 |
| GSM559517 |
HCC-1428/LTED, fulv, rep 2 |
| GSM559518 |
HCC-1428/LTED, fulv, rep 3 |
|
| This SubSeries is part of SuperSeries: |
| GSE37955 |
ERa-dependent E2F transcription can mediate resistance to estrogen deprivation in human breast cancer |
|
| Relations |
| BioProject |
PRJNA128503 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE22533_RAW.tar |
60.7 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...