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| Status |
Public on Jun 01, 2023 |
| Title |
Recruitment of stem-like T cells and resistance to exhaustion support CD4 T cell responses in autoimmunity (scCITE-Seq). |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
Recent findings suggest that undifferentiated, stem-like, antigen specific T cells serve as an important long-term reservoir for autoimmune CD8 T cell responses. However, it is still unclear whether CD4 T cells exhibit a similar differentiation trajectory culminating in terminal differentiation, acquisition of an exhausted phenotype, and loss of stemness and function. We analyzed islet infiltrating T cells by scRNAseq and flow cytometry and found that while CD4 T cells in autoimmune diabetes share many features of exhaustion with CD8 T cells, expression patterns of inhibitory receptors are distinct in autoimmune T cells compared to T cells in chronic LCMV infection.
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| Overall design |
scCITE-Seq
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| Contributor(s) |
Allard D, Raugh A, Perkins B, Aljobaily N, Kolawole E, Liu B, Galland T, Jing Y, Stephens Z, Evavold B, Hale S, Bettini ML, Bettini M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jan 26, 2023 |
| Last update date |
Jun 03, 2023 |
| Contact name |
Maria Bettini |
| E-mail(s) |
Maria.bettini@path.utah.edu
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| Organization name |
University of Utah
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| Street address |
University of Utah
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| City |
Salt Lake City |
| ZIP/Postal code |
84112 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (12)
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| GSM6997587 |
Pancreatic islets T cells, mRNA-derived cDNA, biol rep1 |
| GSM6997588 |
Pancreatic islets T cells, mRNA-derived cDNA, biol rep2 |
| GSM6997589 |
Pancreatic islets T cells, mRNA-derived cDNA, biol rep3 |
| GSM6997590 |
Pancreatic islets T cells, mRNA-derived cDNA, biol rep4 |
| GSM6997591 |
Pancreatic islets T cells, TCR-derived cDNA, biol rep1 |
| GSM6997592 |
Pancreatic islets T cells, TCR-derived cDNA, biol rep2 |
| GSM6997593 |
Pancreatic islets T cells, TCR-derived cDNA, biol rep3 |
| GSM6997594 |
Pancreatic islets T cells, TCR-derived cDNA, biol rep4 |
| GSM6997595 |
Pancreatic islets T cells, ADT-derived cDNA, biol rep1 |
| GSM6997596 |
Pancreatic islets T cells, ADT-derived cDNA, biol rep2 |
| GSM6997597 |
Pancreatic islets T cells, ADT-derived cDNA, biol rep3 |
| GSM6997598 |
Pancreatic islets T cells, ADT-derived cDNA, biol rep4 |
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| Relations |
| BioProject |
PRJNA928482 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE223833_RAW.tar |
171.7 Mb |
(http)(custom) |
TAR (of CSV, MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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