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Series GSE22383 Query DataSets for GSE22383
Status Public on Jun 17, 2010
Title Insulin-like Signaling Determines Survival During Stress via Post Transcriptional Mechanisms in C. elegans.
Organism Caenorhabditis elegans
Experiment type Expression profiling by array
Summary The insulin-like signaling (ILS) pathway regulates metabolism and is known to modulate adult lifespan in C. elegans. Altered stress responses and resistance to a wide range of stressors are also associated with changes in ILS and contribute to enhanced longevity. The transcription factors DAF-16 and HSF-1 are key effectors of the longevity phenotype. We demonstrate that increased intrinsic thermotolerance, due to lower ILS, is not dependent on stress induced HSF-1 transcriptional responses but instead requires active protein translation. Translation profiling experiments reveal genes that are post-transcriptionally regulated in response to altered ILS during heat shock in a DAF-16-dependent manner. Furthermore, several novel proteins are specifically required for ILS effects on thermotolerance. We propose that lowered-ILS results in DAF-16-induced metabolic and physiological changes that precondition a translational response to modulated survival under acute stress.
Overall design 72 experimental samples were analyzed using custom oligo microarrays. A wild type sample pool was used as the Cy3 reference/control for all experimetal samples. All extracted RNA prior to array analysis was fractioned (via a sucrose gradient) based on ribosomal loading and pooled into ribosomal and free RNA (F1), light polysomes (F2) and heavy polysomes (F3) as described in the experimental procedures. The control RNAi is ‘empty’ vector L4440 RNAi feeding vector plasmid (1999 Firelab vector kit) transformed HT115(DE3), which was obtained from the Caenorhabditis Genetics Center (University of Wisconsin).
Contributor(s) McColl G, Rogers AN, Alavez S, Hubbard AE, Melov S, Link CD, Bush AI, Kapahi P, Lithgow GJ
Citation(s) 20816092
Submission date Jun 16, 2010
Last update date Mar 22, 2012
Contact name Simon Melov
Phone 415 209 2068
Organization name Buck Institute for Research on Aging
Lab Melov Lab
Street address 8001 Redwood Blvd
City Novato
State/province CA
ZIP/Postal code 94945
Country USA
Platforms (1)
GPL9458 Washington University/Genome Sequencing Center C. elegans 23K
Samples (72)
GSM556790 Wild type (N2)_on Control RNAi_Fraction1_group1
GSM556791 Wild type (N2)_on Control RNAi_Fraction2_group1
GSM556792 Wild type (N2)_on Control RNAi_Fraction3_group1
BioProject PRJNA127629

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Supplementary file Size Download File type/resource
GSE22383_RAW.tar 146.0 Mb (http)(custom) TAR (of GPR)
Raw data provided as supplementary file
Processed data included within Sample table

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