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| Status |
Public on Nov 02, 2023 |
| Title |
Transcriptional dysregulation impairs efficacy of neural stem cell activation during aging. |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
To study the role of MLL complex in transcriptional dysregulation of NSC aging, we performed ChIP-seq of MLL complex subunits (Kmt2d, Rbbp5, and Wdr5) and Cbx3 in young and aged NSCs to analyze their binding activity at promoters of age-dependent genes.
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| Overall design |
Primary NSCs were isolated from the subventricular zone of the lateral ventricles (SVZ) in the brains of young and aged mice, respectively. The NSCs were maintained in the initial proliferation medium and used in ChIP-sequencing analysis.
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| Contributor(s) |
Li M, Huang C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 26, 2023 |
| Last update date |
Nov 02, 2023 |
| Contact name |
Chengyang Peter Huang |
| E-mail(s) |
chengyhuang@hotmail.com
|
| Organization name |
Shantou University Medical College
|
| Street address |
22 Xinling Street
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| City |
Shantou |
| ZIP/Postal code |
515041 |
| Country |
China |
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| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (24)
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| This SubSeries is part of SuperSeries: |
| GSE212725 |
Transcriptional and epigenetic dysregulation impairs generation of proliferative neural stem and progenitor cells during brain aging |
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| Relations |
| BioProject |
PRJNA928369 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE223781_Aged_Cbx3_peaks.bedGraph.gz |
680.7 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE223781_Aged_Kmt2d_peaks.bedGraph.gz |
234.8 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE223781_Aged_Rbbp5_peaks.bedGraph.gz |
325.2 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE223781_Aged_Wdr5_peaks.bedGraph.gz |
399.4 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE223781_Young_Cbx3_peaks.bedGraph.gz |
786.5 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE223781_Young_Kmt2d_peaks.bedGraph.gz |
350.4 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE223781_Young_Rbbp5_peaks.bedGraph.gz |
297.8 Mb |
(ftp)(http) |
BEDGRAPH |
| GSE223781_Young_Wdr5_peaks.bedGraph.gz |
389.5 Mb |
(ftp)(http) |
BEDGRAPH |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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