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Series GSE223766 Query DataSets for GSE223766
Status Public on Apr 03, 2024
Title Linking CRISPR/Cas9 double-strand break profiles to gene editing precision with BreakTag [48sgRNA]
Organism Homo sapiens
Experiment type Other
Summary Here we developed BreakTag, a versatile, highly parallel and scissile-aware methodology for the profiling of Cas9-induced DNA double strand break (DSBs), to identify molecular determinants influencing Cas9 incisions
 
Overall design Map the off-target landscape of 46 sgRNAs targeting 12 clinically relevant genes with Streptococcus pyogenes (Sp)Cas9 using BreakTag
 
Contributor(s) Longo GM, Sayols S, Roukos V
Citation(s) 38740992
Submission date Jan 26, 2023
Last update date Jul 03, 2024
Contact name Sergi Sayols
Organization name Institute of Molecular Biology, Mainz
Street address Ackermannweg 4
City Mainz
ZIP/Postal code 55128
Country Germany
 
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (48)
GSM6995083 U2OS cells, AAVS1 site 10 gDNA
GSM6995084 U2OS cells, AAVS1 site 11 gDNA
GSM6995085 U2OS cells, AAVS1 site 12 gDNA
This SubSeries is part of SuperSeries:
GSE223772 Linking CRISPR/Cas9 double-strand break profiles to gene editing precision with BreakTag
Relations
BioProject PRJNA928358

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE223766_RAW.tar 6.7 Gb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
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