|
| Status |
Public on Oct 02, 2023 |
| Title |
Dual targeted extracellular vesicles regulating oncogenic genes in pancreatic cancer [Human Array] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Pancreatic ductal adenocarcinoma (PDAC) tumors carry multiple gene mutations and respond poorly to treatments. There is currently an unmet need for drug carriers that can deliver multiple gene cargoes to high solid tumor burden like PDAC. Here, we report a dual-targeted extracellular vesicle (EV) carrying high loads of RNA that effectively suppresses large PDAC tumors in mice. The EV surface contains a CD64 protein that has a tissue targeting peptide and a humanized monoclonal antibody. Cells sequentially transfected with plasmid DNAs encoding for the RNA and protein of interest by Transwell®-based asymmetric cell electroporation released abundant targeted EVs with high RNA loading. Together with a low dose chemotherapy drug, Gemcitabine, dual-targeted EVs effectively suppressed large orthotopic PANC-1 and patient derived xenograft tumors in mice and extended animal survival. This work presents a clinically accessible and scalable way to produce abundant EVs for delivering multiple gene cargoes to large solid tumors.
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| |
|
| Overall design |
HUMAN_arrays
|
| |
|
| Contributor(s) |
Wang K, Lee I, Fallen S, Lee L, Chiang N |
| Citation(s) |
37872156 |
| |
| Submission date |
Jan 20, 2023 |
| Last update date |
Nov 13, 2023 |
| Contact name |
Inyoul Lee |
| Organization name |
Institute for Systems Biology
|
| Street address |
401 Terry Ave. North
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16699 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Feature Number version) |
|
| Samples (15)
|
| GSM6947318 |
Gene expression of Plasmids cd64_TP53 treated BMSC-derived EV _rep3 |
| GSM6947319 |
Gene expression of Plasmids cd64_TP53 treated BMSC-derived EV _rep1 |
| GSM6947320 |
Gene expression of PBS treated BMSC-derived EV _rep2 |
| GSM6947321 |
Gene expression of Control BMSC-derived EV _rep2 |
| GSM6947322 |
Gene expression of Plasmids cd64_TP53 treated BMSC-derived EV _rep2 |
| GSM6947323 |
Gene expression of PBS treated BMSC-derived EV _rep3 |
| GSM6947324 |
Gene expression of PBS treated BMSC-derived EV _rep1 |
| GSM6947325 |
Gene expression of Control BMSC-derived EV _rep1 |
| GSM6947326 |
Gene expression of TP53 treated BMSCs _rep1 |
| GSM6947327 |
Gene expression of Plasmids CD64 and TP53 sequentially treated BMSC-derived EV |
| GSM6947328 |
Gene expression of TP53 treated BMSCs _rep2 |
| GSM6947329 |
Gene expression of Contol BMSCs |
| GSM6947330 |
Gene expression of CD64 treated BMSCs _rep1 |
| GSM6947331 |
Gene expression of PBS treated BMSCs |
| GSM6947332 |
Gene expression of CD64 treated BMSCs _rep2 |
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| This SubSeries is part of SuperSeries: |
| GSE223409 |
Dual targeted extracellular vesicles regulating oncogenic genes in pancreatic cancer |
|
| Relations |
| BioProject |
PRJNA925941 |
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