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| Status |
Public on Jan 16, 2024 |
| Title |
Epitranscriptomics m6A analyses reveal distinct m6A marks in tumor necrosis a (TNF-a)-treated HeLa cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We have performed a epitranscriptomics study in which we first tretaed TNF-alpha in HeLa cells and DMSO was used as a negative control. Total RNA was isolated from TNF-alpha treated cells as well as control cells and apoptotic rate was determined by flow cytometry. Total RNAs were subjected to miCLIP to identify differentially m6A methylated RNAs. We then transcriptionally analyzed apoptotic genes which have differential m6A methylation in TNF-alpha tretament by qPCR. Afterthat, candidate genes were examined at the level of translation by polysome fractioantion assay.
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| Overall design |
Total RNA was isolated from negative control (DMSO) and TNF-alpha treated HeLa cells in triplicate to perform miCLIP assay.
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| Contributor(s) |
Alasar AA, Tuncel O, Saglam B, Gelmez AB, Vatansever IE, Akgul B |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jan 18, 2023 |
| Last update date |
Jan 16, 2024 |
| Contact name |
Bünyamin Akgül |
| E-mail(s) |
bunyaminakgul@iyte.edu.tr
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| Organization name |
Izmir Institute of Technology
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| Department |
Molecular Biology and Genetics
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| Lab |
Non-coding RNA Laboratory
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| Street address |
Gulbahce Campus
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| City |
Izmir |
| State/province |
Urla |
| ZIP/Postal code |
35433 |
| Country |
Turkey |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA925217 |
