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| Status |
Public on Sep 20, 2023 |
| Title |
Bulk RNA-seq of CRISPRi ADGRG6-kd iAT2 following cigarette smoke exposure |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We performed bulk transcriptomic profiling of induced human pluripotent stem cells (iPSCs)-derived type 2 alveolar epithelial cells (iAT2). iPSCs stably expressed CRISPRi (dCas9-KRAB) under the control of doxycyline. iAT2s were transduced with a lentivirus expressing gRNA targeting the transcriptional start site of ADGRG6. Cells were treated with or without doxycyline to intiate CRISPRi-knockdown. Cells were plated at an air-liquid interface, then subsequently exposed to air or 5% cigarette smoke using a VitroCell smoke robot. Cells were harvested for bulk RNA sequencing 8 hours post cigarette smoke exposure
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| Overall design |
Bulk transcriptomic analysis of iPSC-derived type 2 alveolar epithelial cells (iAT2) comparing wild-type cells with CRISPRi-mediated knockdown of ADGRG6 following exposure to air or 5% cigarette smoke exposure
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| Contributor(s) |
Werder RB, Wilson A, Bawa P |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 17, 2023 |
| Last update date |
Sep 20, 2023 |
| Contact name |
Pushpinder Bawa |
| E-mail(s) |
bpushpin@bu.edu
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| Organization name |
Center for Regenerative Medicine
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| Department |
Boston University Medical Campus
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| Street address |
670 Albany St
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| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02118 |
| Country |
USA |
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| Platforms (1) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA924805 |
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