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Series GSE22292 Query DataSets for GSE22292
Status Public on Jun 12, 2010
Title Gene expression in mouse neonatal cardiomyocytes, cardiac fibroblasts, reprogramming failed GFP- cells, and GFP+ iCMs
Organism Mus musculus
Experiment type Expression profiling by array
Summary The reprogramming of fibroblast cells to induced pluripotent stem (iPS) cells raises the possibility that a somatic cell could be reprogrammed to an alternative differentiated fate without first becoming a stem/progenitor cell. A large pool of fibroblast cells exists in the post-natal heart, yet no single “master regulator” of direct cardiac reprogramming has been identified. Here, we report that a combination of three developmental transcription factors (i.e., Gata4, Mef2c and Tbx5) rapidly and efficiently reprogrammed post-natal cardiac or tail-tip fibroblasts directly into differentiated cardiomyocyte-like cells. Induced cardiomyocytes expressed cardiac-specific markers, had a global gene expression profile similar to cardiomyocytes, and contracted spontaneously. Fibroblast cells transplanted into mouse hearts one day after transduction of the three factors also differentiated into cardiomyocyte-like cells. These findings demonstrate that functional cardiomyocytes can be directly reprogrammed from differentiated somatic cells by defined factors. Reprogramming of endogenous or explanted fibroblast cells might provide a source of cardiomyocytes for regenerative approaches.
Overall design To compare the progressive global gene expression pattern of iCMs, cardiomyocytes and cardiac fibroblasts, we isolated RNA from mouse neonatal cardiomyocytes, cardiac fibroblasts, reprogramming failed GFP- cells and GFP+ iCMs, and profiled mRNA expressions by microarray analyses. Arrays were performed using Affymetrix mouse Gene 1.0 ST arrays. Analysis was performed on three biological replicates for each group.
Contributor(s) IEDA M, SRIVASTAVA D
Citation(s) 20691899
Submission date Jun 10, 2010
Last update date Mar 04, 2019
Contact name Masaki Ieda
Phone 415-734-2880
Organization name Gladstone Institute
Street address 1650 Owens street,
City San Francisco
State/province CA
ZIP/Postal code 94404
Country USA
Platforms (1)
GPL6246 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]
Samples (18)
GSM554203 2 week reprogramming failed GFP- cells-rep1
GSM554555 2 week reprogramming failed GFP- cells-rep2
GSM554556 2 week reprogramming failed GFP- cells-rep3
BioProject PRJNA127407

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE22292_RAW.tar 77.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
Raw data provided as supplementary file

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