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| Status |
Public on Jun 11, 2024 |
| Title |
Single cell RNAseq of hepatocytes isolated from UBC-injected mice at different timepoints post-HDTV |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Single-cell sequencing of mVenus-expressing hepatocytes from mouse livers following introduction of either control or oncogenic NRAS-containing transposons
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| Overall design |
Multiplexed single-cell RNAseq experiment: Ten mice were used in this experiment, all having received hydrodynamic tail vein (HDTV) injection of either mVenus-NRAS^G12V-containing or control mVenus only transposons. At different timepoints post-injection (Day 12, Day 30 and Day 218 – after formation of liver tumours detectable by ultrasound imaging), livers were harvested and dissociated to single cells. Mvenus-expressing hepatocytes were flow sorted from the dissociated livers and labelled with Hashtag oligos before multiplexing and single cell RNA sequencing.
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| Contributor(s) |
Chan AS, Narita M |
| Citation(s) |
39112713 |
| |
| Submission date |
Jan 06, 2023 |
| Last update date |
Sep 17, 2024 |
| Contact name |
Adelyne SL Chan |
| E-mail(s) |
Adelyne.Chan@cruk.cam.ac.uk
|
| Organization name |
University of Cambridge
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| Department |
Cancer Research UK
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| Lab |
Narita Lab
|
| Street address |
Li Ka Shing Way
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0RE |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE222951 |
Titration of RAS alters senescent state and influences tumour initiation |
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| Relations |
| BioProject |
PRJNA919040 |
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