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Series GSE222051 Query DataSets for GSE222051
Status Public on Apr 04, 2023
Title Transcriptional analysis for off-target effects by sgRNA variants in E.coli BL21(DE3)
Organism Escherichia coli
Experiment type Expression profiling by high throughput sequencing
Summary Five different sgRNAs targeting mCherry gene (wild-type, without a spacer, and three truncated variants) were used to investigate the off-target effects of CRISPR interference.
 
Overall design Total RNA sequencing data from E. coli expressing CRISPRi system targeting mCherry gene with synthetic sgRNAs
Web link https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/nar/51/9/10.1093_nar_gkad234/1/gkad234_supplemental_file.pdf?Expires=1691549892&Signature=OVs2JHyLuHQjqasUiJYt~ZEyl3q8FHMZ6bZ87q48CCkQ6LamT79CcgvikxHSOCCHskXx8Ens0Qh9qNEuuTICym00bp1zPFrVtl1cR331nO2jx8GERHxryb71LNfmwCnsKhzpWz4gBhIkBJvobovZkbTC1b-nZ10qrqZrSTGNI4agGiTMhbgFqpqZm5UnDlsZmtQiBaxbDhv6gnyEqkfmVqj0qI4ClpPbYWNaxfs9hpFOp6hmsIHKUn0pUSgJ3GtTovfUUkx1Q3NWAZq7pB3uj-K3G3kuRU4tZC9mHii4CQRqrDPUmem~fFVfOR5~VWlJM6Os4pGC50vYj1ibL9eD-w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA
 
Contributor(s) Byun G, Yang J, Seo SW
Citation(s) 36999618
Submission date Jan 03, 2023
Last update date Jul 04, 2023
Contact name Sang Woo Seo
Organization name Seoul National University
Street address 1, Gwanak-ro, Gwanak-gu
City Seoul
ZIP/Postal code 08826
Country South Korea
 
Platforms (1)
GPL28126 NextSeq 550 (Escherichia coli)
Samples (10)
GSM6912723 sg_PC_rep1
GSM6912724 sg_PC_rep2
GSM6912725 sg_WT_rep1
Relations
BioProject PRJNA917517

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Supplementary file Size Download File type/resource
GSE222051_RAW.tar 2.3 Mb (http)(custom) TAR (of GTF)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
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