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Series GSE22182 Query DataSets for GSE22182
Status Public on Dec 31, 2010
Title Global Deterministic and Stochastic Allelic Specific Gene Expression in Single Blastomeres of Mouse Early Embryos
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Here we modified a single cell whole transcriptome amplification method to make it capable of amplifying cDNAs as long as 3kb efficiently and unbiasedly. We combined this modified single cell cDNA amplification method with Applied Biosystems next generation sequencing SOLiD™ System to set up a single cell whole transcriptome assay. The modified amplification strategy allows us to amplify full-length cDNAs for most of the expressed genes. We show that it is feasible to get digital gene expression profiles at single cell resolution. This allows us to ask fundamental biological questions that could not be addressed previously and to understand transcriptome complexity at the resolution of a single cell.
Oocyte, Two-cell, Four-cell, and 8-cell stage embryos were recovered from MF1 females mated with MF1 male mice (Nagy et al. 2003). The zona pellucida was removed by treatment with acidic tyrode solution. The individual blastomeres were separated by gentle pipeting using a glass capillary.
 
Overall design RNA-Seq from 24 single mouse blastomeres from oocytes, 2-cell, 4-cell and 8-cell stages.
 
Contributor(s) Tang F, Barbacioru C, Lao K, Surani MA
Citation(s) 21731673
Submission date Jun 07, 2010
Last update date May 15, 2019
Contact name Catalin Barbacioru
E-mail(s) catalin@appliedbiosystems.com
Organization name Applied Biosystems
Street address 850 Lincoln Centre Dr
City Foster City
State/province CA
ZIP/Postal code 94404
Country USA
 
Platforms (1)
GPL9318 AB SOLiD System 3.0 (Mus musculus)
Samples (24)
GSM552323 wild type mouse oocyte #1_WT_A7
GSM552324 wild type mouse oocyte #2_WT_10
GSM552325 mouse Dicer knockout oocyte #3_Dicer_2A
Relations
SRA SRP002609
BioProject PRJNA128815

Download family Format
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Supplementary file Size Download File type/resource
GSE22182_RAW.tar 4.9 Gb (http)(custom) TAR (of SRF)
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA
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