|
| Status |
Public on Feb 23, 2023 |
| Title |
Transcriptomic characterization of control and drug-treated APOB-mutant organoids to study steatosis-resolving drug effects |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We treated APOB-mutant organoids with different anti-NAFLD drug candidates that were effective at resolving steatosis. To understand the mechanisms of drug action and adverse drug effects we performed bulk RNA-sequencing on drug-treated and vehicle-treated APOB mutant organoids.
|
| |
|
| Overall design |
APOB-mutant organoids, 2 clonal lines from 2 different donors, were treated with the respective drug (ACCi, FASi, DGAT2i, FXRa, or hFGF19) or vehicle (control) for 7 days. Thereafter, organoids were harvested for RNA extraction.
|
| |
|
| Contributor(s) |
Delilah H, Benedetta A, Hans C |
| Citation(s) |
36823355 |
| |
| Submission date |
Dec 23, 2022 |
| Last update date |
Jun 01, 2023 |
| Contact name |
Delilah Hendriks |
| Organization name |
Hubrecht Institute
|
| Street address |
Uppsalalaan 8
|
| City |
Utrecht |
| ZIP/Postal code |
3584 CT |
| Country |
Netherlands |
| |
|
| Platforms (1) |
|
| Samples (12)
|
| GSM6893374 |
ACCi treated APOB-/- organoids from donor 1 |
| GSM6893375 |
vehicle treated APOB-/- organoids from donor 1 |
| GSM6893376 |
DGAT2i treated APOB-/- organoids from donor 1 |
| GSM6893377 |
FASi treated APOB-/- organoids from donor 1 |
| GSM6893378 |
FGF19 treated APOB-/- organoids from donor 1 |
| GSM6893379 |
ACCi treated APOB-/- organoids from donor 2 |
| GSM6893380 |
vehicle treated APOB-/- organoids from donor 2 |
| GSM6893381 |
DGAT2i treated APOB-/- organoids from donor 2 |
| GSM6893382 |
FASi treated APOB-/- organoids from donor 2 |
| GSM6893383 |
FGF19 treated APOB-/- organoids from donor 2 |
| GSM6893384 |
FXRa treated APOB-/- organoids from donor 1 |
| GSM6893385 |
FXRa treated APOB-/- organoids from donor 2 |
|
| Relations |
| BioProject |
PRJNA915399 |
Less...
More...