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Series GSE22148 Query DataSets for GSE22148
Status Public on Jun 08, 2010
Title Induced Sputum Genes Associated With Spirometroc and Radiological Disease Severity in COPD Ex-smokers
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Induced sputum is used to sample inflammatory cells, predominantly neutrophils and macrophages, from the airways of COPD patients. Our aim was to identify candidate genes associated with the degree of airflow obstruction and the extent of emphysema by expression profiling, and then to confirm these findings for selected candidates using specific PCR and protein analysis.
 
Overall design Two sputum studies were performed in GOLD stage 2 -4 COPD ex-smokers from the ECLIPSE cohort. First, gene array profiling at baseline in 1480 patients was performed. At year 1, samples from a separate population of 176 patients were used for real-time PCR. The gene expression findings for IL-18R were further analysed using immunohistochemistry in lung tissue and induced sputum samples from patients outside the ECLIPSE cohort.
 
Contributor(s) Singh D, Fox S, Tal-Singer R, Plumb J, Bates S, Broad P, Riley J, Celli B
Citation(s) 21441172
Submission date Jun 04, 2010
Last update date Mar 25, 2019
Contact name steve matthew fox
E-mail(s) steven.m.fox@gsk.com
Phone 01438 763226
Organization name GlaxoSmithKline
Department Statistics
Street address Gunnels Wood Road
City Stevenage
ZIP/Postal code SG1 2NY
Country United Kingdom
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (143)
GSM550785 Sputum from Stage II Moderate, biological replicate 1
GSM550786 Sputum from Stage II Moderate, biological replicate 2
GSM550787 Sputum from Stage III Severe, biological replicate 1
Relations
BioProject PRJNA127563

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE22148_RAW.tar 732.1 Mb (http)(custom) TAR (of CEL)
SRA Run SelectorHelp
Raw data provided as supplementary file
Processed data included within Sample table

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