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| Status |
Public on Jun 05, 2024 |
| Title |
ATAC-seq analyses of AR-V7. |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Although AR-V7 has been intensively studied, it remains unclear whether AR-V7 and other AR splicing variants can specifically activate a distinctive transcriptional program from the full-length AR (AR-FL), and whether AR-V7 may play a role in accelerating the metastatic progression of castration-resistant PCa (CRPC). In this study, we hypothesize that AR-V7 can drive a distinct transcription program from AR-FL in CRPC condition. To test this, we performed ATAC-seq using LNCaP model with inducible overexpression of AR-V7 to examine the function of AR-V7. We demonstrated that AR-V7 has “pioneer factor-like” activities to access the androgen-responsive elements (AREs) located at compact chromatin regions. More importantly, we found that SOX9, a critical metastasis driver gene, was a direct target and downstream effector of AR-V7, and its protein expression was dramatically upregulated at AR-V7-induced bone lesions.
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| Overall design |
ATAC-seq analyses in LNCaP table cells with or without overexpression of AR-V7 and stimulated with or without DHT under 5% charcoal-stripped FBS condition. The stable cells were generated by stably infecting LNCaP cells with tetracycline inducible overexpression of wild-type AR-V7 virus. These stable cells were grown in the medium containing 10% tetracycline-free FBS.
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| Contributor(s) |
Han D, Labaf M, Zarringhalam K, Cai C |
| Citation(s) |
38687617 |
| |
| Submission date |
Dec 16, 2022 |
| Last update date |
Jun 05, 2024 |
| Contact name |
Maryam Labaf |
| E-mail(s) |
maryam.labaf001@umb.edu
|
| Organization name |
University of Massachusetts Boston
|
| Department |
Biology
|
| Lab |
Cai Lab
|
| Street address |
100 William T Morrissey Blvd
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02125 |
| Country |
USA |
| |
|
| Platforms (1) |
|
| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE221142 |
Distinct activity of androgen receptor splice variants in promoting prostate cancer metastasis |
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| Relations |
| BioProject |
PRJNA913099 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE221137_ATAC_LN-tet-ARV7_CSS_DHT.bw |
200.9 Mb |
(ftp)(http) |
BW |
| GSE221137_ATAC_LN-tet-ARV7_CSS_DHT.narrowPeak.gz |
2.6 Mb |
(ftp)(http) |
NARROWPEAK |
| GSE221137_ATAC_LN-tet-ARV7_CSS_Dox.bw |
210.9 Mb |
(ftp)(http) |
BW |
| GSE221137_ATAC_LN-tet-ARV7_CSS_Dox.narrowPeak.gz |
2.1 Mb |
(ftp)(http) |
NARROWPEAK |
| GSE221137_ATAC_LN-tet-ARV7_CSS_Dox_DHT.bw |
404.0 Mb |
(ftp)(http) |
BW |
| GSE221137_ATAC_LN-tet-ARV7_CSS_Dox_DHT.narrowPeak.gz |
1.9 Mb |
(ftp)(http) |
NARROWPEAK |
| GSE221137_ATAC_LN-tet-ARV7_CSS_Veh.bw |
318.2 Mb |
(ftp)(http) |
BW |
| GSE221137_ATAC_LN-tet-ARV7_CSS_Veh.narrowPeak.gz |
2.3 Mb |
(ftp)(http) |
NARROWPEAK |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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