|
| Status |
Public on Aug 03, 2010 |
| Title |
Mammalian microRNAs predominantly act to decrease target mRNA levels |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Expression profiling by array
|
| Summary |
MicroRNAs (miRNAs) are endogenous ~22-nucleotide RNAs that mediate important gene-regulatory events by pairing to the mRNAs of protein-coding genes to direct their repression. Repression of these regulatory targets leads to decreased translational efficiency and/or decreased mRNA levels, but the relative contributions of these two outcomes have been largely unknown, particularly for endogenous targets expressed at low-to-moderate levels. Here, we use ribosome profiling to measure the overall effects on protein production and compare these to simultaneously measured effects on mRNA levels. For both ectopic and endogenous miRNA regulatory interactions, lowered mRNA levels account for most (≥84%) of the decreased protein production. These results show that changes in mRNA levels closely reflect the impact of miRNAs on gene expression and indicate that destabilization of target mRNAs is the predominant reason for reduced protein output.
This SuperSeries is composed of the SubSeries listed below.
|
| |
|
| Overall design |
Refer to individual Series.
|
| |
|
| Citation(s) |
20703300 |
| Submission date |
May 26, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Huili Guo |
| E-mail(s) |
hguo@nus.edu.sg
|
| Organization name |
National University of Singapore
|
| Street address |
28 Medical Drive
|
| City |
Singapore |
| ZIP/Postal code |
117456 |
| Country |
Singapore |
| |
|
| Platforms (4)
|
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
|
| Samples (24)
|
| GSM546923 |
mRNASeq_miR-155_32hr_runs1-3 |
| GSM546924 |
Footprint_miR-1_32hr_runs1-2 |
| GSM546925 |
mRNASeq_miR-1_32hr_runs1-3 |
| GSM546926 |
Footprint_mock_12hr_run1 |
| GSM546927 |
mRNASeq_mock_12hr_runs1-2 |
| GSM546928 |
Footprint_miR-155_12hr_run1 |
| GSM546929 |
mRNASeq_miR-155_12hr_runs1-2 |
| GSM546930 |
Footprint_miR-1_12hr_run1 |
| GSM546931 |
mRNASeq_miR-1_12hr_runs1-2 |
| GSM546987 |
Footprint_wild-type_runs1-2 |
| GSM546988 |
mRNASeq_wild-type_runs1-3 |
| GSM546989 |
Footprint_mir-223-knockout_runs1-2 |
| GSM546990 |
mRNASeq_mir-223-knockout_runs1-3 |
| GSM546991 |
HeLa cells, mock-transfected, 32hr |
| GSM546992 |
HeLa cells, transfected with miR-155 duplex, 32hr |
| GSM546993 |
HeLa cells, transfected with miR-1 duplex, 32hr |
| GSM546994 |
HeLa cells, mock-transfected, 12hr |
| GSM546995 |
HeLa cells, transfected with miR-155 duplex, 12hr |
| GSM546996 |
HeLa cells, transfected with miR-1 duplex, 12hr |
| GSM546997 |
Neutrophils cultured from wild-type mouse bone marrow |
| GSM546998 |
Neutrophils cultured from mir-223 knockout mouse bone marrow |
|
| This SuperSeries is composed of the following SubSeries:
|
| GSE21992 |
Analysis of HeLa cells after transfection with miR-1 or miR-155, by ribosome profiling and mRNA-Seq |
| GSE22001 |
Analysis of mir-223 knockout cultured neutrophils versus wild-type cultured neutrophils, by ribosome profiling and mRNA-Seq |
| GSE22002 |
Analysis of HeLa cells after transfection with miR-1 or miR-155, by microarray profiling |
| GSE22003 |
Analysis of mir-223 knockout cultured neutrophils versus wild-type cultured neutrophils, by microarray profiling |
|
| Relations |
| BioProject |
PRJNA127467 |
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