|
| Status |
Public on May 24, 2023 |
| Title |
CRISPR-based epigenome editing screens identify transcriptional and epigenetic regulators of human CD8 T cell function [CRISPRi/a TF scRNA-seq characterization] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
Human CD8+CCR7+ T cells from three donors were transduced with CRISPRi and CRISPRa TF gRNA libraries and expanded for 10 days. After 10 days, we used single-cell RNA sequencing (scRNA-seq) to analyze the transcriptomic effects of silencing or activating each gene candidate.
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| |
|
| Overall design |
The transcriptome profiles of cells with the same targeting gRNA were compared to cells with only non-targeting gRNAs.
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| |
|
| Contributor(s) |
McCutcheon SR, Gersbach CA |
| Citation(s) |
37205457, 37945901 |
| |
| Submission date |
Nov 29, 2022 |
| Last update date |
Dec 14, 2023 |
| Contact name |
Sean McCutcheon |
| E-mail(s) |
sean.mccutcheon@duke.edu
|
| Phone |
7073447178
|
| Organization name |
Duke University
|
| Department |
Biomedical Engineering
|
| Lab |
Charles Gersbach
|
| Street address |
101 Science Drive, CIEMAS Rm. 2323
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27708 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (6)
|
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| This SubSeries is part of SuperSeries: |
| GSE218988 |
CRISPR-based epigenome editing screens identify transcriptional and epigenetic regulators of human CD8 T cell function |
|
| Relations |
| BioProject |
PRJNA906519 |
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