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| Status |
Public on Nov 29, 2022 |
| Title |
PD-L1 regulates inflammatory programs of macrophages from human pluripotent stem cells. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
CRISPR knock-out combined with flow cytometry and scRNA-seq show that PD-L1 ablation compromises macrophage development from human pluripotent stem cells
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| Overall design |
PD-L1 is detected to be sharply expressed in only macrophages, and through PD-L1 knock-out by CRISPR, we found a surge decrease of macrophage population developed from human pluripotent stem cells, by flow cytometry. ScRNA-seq of wild-type and PD-L1 knock-out sample was used to characterize the difference of macrophages between them.
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| Web link |
https://pubmed.ncbi.nlm.nih.gov/37949473/
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| Contributor(s) |
Sugimura R, Cao H, Xiang D, Zhang S |
| Citation(s) |
37949473 |
| |
| Submission date |
Nov 25, 2022 |
| Last update date |
Dec 05, 2023 |
| Contact name |
Shihui ZHANG |
| E-mail(s) |
shihuizhang974@gmail.com
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| Organization name |
Centre for Translational Stem Cell Biology Limited
|
| Street address |
Science and Technology W Ave
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| City |
Hong Kong |
| ZIP/Postal code |
999077 |
| Country |
China |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA905351 |
