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| Status |
Public on Nov 10, 2023 |
| Title |
OGFOD1 Regulates Translation to Alter Hypertrophy Susceptibility |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Cardiac hypertrophy, a precursor for heart failure, requires increased translation. However, little is known of the mechanisms that regulate translation in hypertrophy. Members of the 2-oxoglutarate-dependent dioxygenase family regulate several aspects of gene expression, including translation. An important member of this family is OGFOD1. Here, we show OGFOD1 accumulates in failing human hearts. Upon OGFOD1 deletion, murine hearts showed transcriptomic and proteomic changes, with only 21 factors (0.6%) changing in the same direction at both the mRNA and protein levels. Additionally, OGFOD1-KO mice were protected from induced hypertrophy, supporting a role for OGFOD1 in the cardiac response to chronic stress.
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| Overall design |
WT and OGFOD1-KO mice aged 3-5 months were treated with either vehicle (1X PBS and Sodium Ascorbate) or isoproterenol (1X PBS, Sodium Ascorbate, and Isoproterenol) for 2 weeks by osmotic minipump. Hearts were isolated from these mice and RNA was extracted and subjected to RNAsequencing.
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| Contributor(s) |
Kennedy LM, Murphy E, Liu P, Luo Y, Tunc I, Pirooznia M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 10, 2022 |
| Last update date |
Nov 10, 2023 |
| Contact name |
Sonja Best |
| E-mail(s) |
sbest@niaid.nih.gov
|
| Phone |
406-375-9694
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| Organization name |
National Institutes of Health
|
| Department |
National Institute of Allergy and Infectious Diseases
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| Lab |
Laboratory of Neurological Infections and Immunity
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| Street address |
903 SOUTH 4TH STREET RM 3203
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| City |
Hamilton |
| State/province |
MT |
| ZIP/Postal code |
59840 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA900269 |
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