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| Status |
Public on May 08, 2010 |
| Title |
The ubiquitin-conjugating enzyme HR6B is required for maintenance of X chromosome silencing in mouse spermatocytes and spermatids |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Background: The ubiquitin-conjugating enzyme HR6B is required for spermatogenesis in mouse. Loss of HR6B results in aberrant histone modification patterns on the trancriptionally silenced X and Y chromosomes (XY body) and on centromeric chromatin in meiotic prophase. We studied the relationship between these chromatin modifications and their effects on global gene expression patterns, in spermatocytes and spermatids.
Results: HR6B is enriched on the XY body and on centromeric regions in pachytene spermatocytes. Global gene expression analyses revealed that spermatid-specific single- and multicopy X-linked genes are prematurely expressed in Hr6b knockout spermatocytes. Very few other differences in gene expression were observed in these cells, except for upregulation of major satellite repeat transcription. In contrast, in Hr6b knockout spermatids, 7298 genes were differentially expressed; 65% of these genes was downregulated, but we observed a global upregulation of gene transcription from the X chromosome. In wildtype spermatids, approximately 20% of the single-copy X-linked genes reach an average expression level that is similar to the average expression from autosomes.
Conclusions: Spermatids maintain an enrichment of repressive chromatin marks on the X chromosome, originating from meiotic prophase, but this does not interfere with transcription of the single-copy X-linked genes that are reactivated or specifically activated in spermatids. HR6B represses major satellite repeat transcription in spermatocytes, and functions in the maintenance of X chromosome silencing in spermatocytes and spermatids. It is discussed that these functions involve modification of chromatin structure, possibly including H2B ubiquitylation.
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| Overall design |
8 Affymetrix microarrays were generated: 4 from wildtype and HR6B knockout spermatocytes (2 replicates each), and 4 from wildtype and HR6B knockout spermatids (2 replicates each).
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| Contributor(s) |
Achame EM, Wassenaar E, Hoogerbrugge JW, Sleddens-Linkels E, Ooms M, Sun Z, van IJcken WF, Grootegoed JA, Baarends WM |
| Citation(s) |
20537150 |
| Submission date |
May 07, 2010 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Eskeatnaf Mulugeta Achame |
| E-mail(s) |
e.mulugetaachame@erasmusmc.nl
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| Phone |
0031614883537
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| URL |
http://www.erasmusmc.nl/rede/
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| Organization name |
Erasmusmc
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| Department |
Reproduction and Development
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| Lab |
Reproduction and Development
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| Street address |
Dr Molewaterplein 50
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| City |
Rotterdam |
| State/province |
Rotterdam |
| ZIP/Postal code |
3015 GE |
| Country |
Netherlands |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (8)
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| GSM542304 |
Late Spermatocyte, HR6B Knockout, rep1 |
| GSM542305 |
Late Spermatocyte, HR6B Knockout, rep2 |
| GSM542306 |
Late Spermatocyte, Wildtype, rep1 |
| GSM542307 |
Late Spermatocyte, Wildtype, rep2 |
| GSM542308 |
Round Spermatid, HR6B Knockout, rep1 |
| GSM542309 |
Round Spermatid, HR6B Knockout, rep2 |
| GSM542310 |
Round Spermatid, Wildtype, rep1 |
| GSM542311 |
Round Spermatid, Wildtype, rep2 |
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| Relations |
| BioProject |
PRJNA127337 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE21749_RAW.tar |
29.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Raw data provided as supplementary file |
| Processed data included within Sample table |
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