|
| Status |
Public on May 31, 2023 |
| Title |
The Chd4 helicase regulates chromatin accessibility and gene expression critical for β-cell function in vivo [ATAC-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
To establish the role of Chd4 on controlling gene expression programs in the beta cell, we generated a tamoxifen-inducible, beta-cell-specific knockout mouse model; control (MIP-CreERT; Chd4flox/+;R26RtdTomato) and Chd4Db (MIP-CreERT; Chd4flox/flox;R26RtdTomato.
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| |
|
| Overall design |
Dispersed islets from tamoxifen treated control and Chd4Db mice were flow-sorted, and ATAC-Seq performed.
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| |
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| Contributor(s) |
Spaeth J, Davidson R |
| Citation(s) |
36913741 |
| |
| Submission date |
Nov 07, 2022 |
| Last update date |
Jun 01, 2023 |
| Contact name |
Jason Spaeth |
| E-mail(s) |
jspaeth@iu.edu
|
| Phone |
3172748986
|
| Organization name |
Indiana University
|
| Department |
Pediatrics
|
| Lab |
MS2021
|
| Street address |
635 Barnhill Dr.
|
| City |
Indianapolis |
| State/province |
IN |
| ZIP/Postal code |
46202 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE217446 |
The Chd4 helicase regulates chromatin accessibility and gene expression critical for β-cell function in vivo. |
|
| Relations |
| BioProject |
PRJNA899040 |
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