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| Status |
Public on Apr 01, 2024 |
| Title |
Multiome (GEX + ATAC) profile at single cell level of Foxf1 positive endothelial cells and mesenchymal cells from mouse lung at E18.5 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Foxf1 is enriched and differentially expressed in lung cells derived from mesoderm. We used single cell RNA sequencing (snRNAseq + snATAC) to analyze the dynamic transcriptional regulation in various lung cell types. The single cell analysis show the cell specific regulation of Foxf1 by distal enhancers that contribute to the molecular pathology of ACDMPV.
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| Overall design |
Foxf1 positive cells from lung of mice were isolated by Fluorescence-activated cell sorting (FACS) according to the presence or absence of GFP signal and analyzed using 10X multiome-seq. The cells derived from mESC cells via blastocyst complementation are processed and analysed by single cell RNA sequencing.
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| Contributor(s) |
Wang G, Kalinichenko V |
| Citation(s) |
38898031 |
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| Submission date |
Nov 03, 2022 |
| Last update date |
Jul 01, 2024 |
| Contact name |
Guolun Wang |
| Organization name |
Cincinnati Children's Hospital Medical Center
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| Street address |
3333 Burnet Avenue
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| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA897893 |
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