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Series GSE21664 Query DataSets for GSE21664
Status Public on May 10, 2010
Title Gene expression data of interleukin-7-dependent and BCR-ABL1-transformed pre-B cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary This analysis focused on identifying factors that protect pre-B cells against DNA double strand break (DSB)-mediated DNA damage stress during pre-B cell differentiation. Differentiation of pre-B cells including immunoglobulin light chain gene recombination were performed by withdrawal of interleukin-7 (IL-7) from IL-7-dependent murine pre-B cells or by inhibition of the BCR-ABL1 kinase activity in BCR-ABL1-transformed pre-B cells.
The BCR-ABL1 kinase inhibitor STI571 (Imatinib) was used for the inhibition of BCR-ABL1.
Overall design IL-7-dependent murine pre-B cells were either cultured in IL-7 (10 ng/ml) or induced to differentiate by withdrawal of IL-7. BCR-ABL1-transformed pre-B cells were either treated with 10 µM STI571 (in absence or presence of 10 ng/ml IL-7) for 16 hours or cultured without STI571. Three samples for each condition were processed.
Contributor(s) Duy C, Yu JJ, Nahar R, Swaminathan S, Kweon S, Polo JM, Valls E, Klemm L, Shojaee S, Cerchietti L, Schuh W, Jäck H, Hurtz C, Ramezani-Rad P, Herzog S, Jumaa H, Koeffler PH, de Alborán IM, Melnick AM, Ye HB, Müschen M
Citation(s) 20498019
Submission date May 04, 2010
Last update date May 04, 2018
Contact name Markus Müschen
Phone + 1 323 361 5592
Fax +1 323 361 1516
Organization name University of Southern California
Department Childrens Hospital Los Angeles
Lab Müschen Lab
Street address 4650 Sunset Blvd
City Los Angeles
State/province CA
ZIP/Postal code 90027
Country USA
Platforms (1)
GPL8321 [Mouse430A_2] Affymetrix Mouse Genome 430A 2.0 Array
Samples (15)
GSM540647 IL-7-dependent pre-B cells - withdrawal of IL-7 rep 1
GSM540648 IL-7-dependent pre-B cells - withdrawal of IL-7 rep 2
GSM540649 IL-7-dependent pre-B cells - withdrawal of IL-7 rep 3
BioProject PRJNA125901

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Supplementary file Size Download File type/resource
GSE21664_RAW.tar 27.0 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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