GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE21520 Query DataSets for GSE21520
Status Public on Feb 18, 2011
Title The gene regulatory cascade linking proneural specification with differentiation in Drosophila sensory neurons
Organism Drosophila melanogaster
Experiment type Expression profiling by array
Summary A challenge in systems biology is to understand the gene regulatory networks that connect early cellular specification to terminal differentiation of specific cell types. In neurogenesis, neural specification has been well studied, but the link between the proneural transcriptional regulators of specification and the genes that must be activated to construct differentiated neurons is obscure. High resolution temporal profiling of gene expression reveals the events downstream of Atonal (Ato) proneural gene function in Drosophila sensory neurons. Unexpectedly, many differentiation genes are activated soon after specification, even before cell cycle exit and overt neuronal differentiation. Prominent among them are genes required for construction of the ciliary dendrite. Ato activates differentiation both directly and indirectly via several intermediate transcriptional regulators, including Rfx and a new Forkhead family factor. Our analysis of these factors and their regulation provides insight into how proneural factors regulate neuronal subtype differentiation.
Investigating the molecular mechanisms of peripheral nervous sytem development in Drosophila melanogaster.
Overall design Affymetrix Drosophila version 2.0 chips used to measure gene expression from GFP+ and GFP- cells from embryos expressing GFP under the control of the atonal gene enhancer in both wild type and mutant embryos. Data generated for three developmental time-points in quadruplicate.
Contributor(s) Simpson TI, Cachero S, ZurLage PI, Jarman AP
Citation(s) 21283833
Submission date Apr 26, 2010
Last update date Aug 28, 2018
Contact name Ian Simpson
Organization name University of Edinburgh
Department Institute for Adaptive and Neural Computation
Lab School of Informatics
Street address 10 Crichton Street
City Edinburgh
ZIP/Postal code EH8 8AB
Country United Kingdom
Platforms (1)
GPL1322 [Drosophila_2] Affymetrix Drosophila Genome 2.0 Array
Samples (31)
GSM537542 atonalGFP+ t1 rep1
GSM537543 atonalGFP- t1 rep1
GSM537544 atonalGFP+ t1 rep2
BioProject PRJNA125961

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE21520_RAW.tar 92.8 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap