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Series GSE214578 Query DataSets for GSE214578
Status Public on Oct 05, 2022
Title Estrogen receptor alpha mutations regulate gene expression and cell growth in breast cancer through microRNAs (NanoString)
Organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary Estrogen receptor α (ER) mutations occur in up to 30% of metastatic ER-positive breast cancers. Recent data has shown that ER mutations impact the expression of thousands of genes not typically regulated by wildtype ER. While the majority of these altered genes can be explained by constant activity of mutant ER or genomic changes such as altered ER binding and chromatin accessibility, as much as 33% remain unexplained, indicating the potential for post-transcriptional effects. Here we explored the role of microRNAs in mutant ER-driven gene regulation and identified several microRNAs that are dysregulated in ER mutant cells. These differentially regulated microRNAs target a significant portion of mutant-specific genes involved in key cellular processes. When the activity of microRNAs is altered using mimics or inhibitors, significant changes are observed in gene expression and cellular proliferation related to mutant ER. An in-depth evaluation of miR-301b led us to discover an important role for PRKD3 in the proliferation of ER mutant cells. Our findings show that microRNAs contribute to mutant ER gene regulation and cellular effects in breast cancer cells.
 
Overall design microRNA profiles from T-47D breast cancer ESR1 mutant or WT cell lines and from ESR1 mutant or WT breast cancer patient derived xenograft (PDX) models
 
Contributor(s) Arnesen S, Gertz J
Citation(s) 37275275
Submission date Sep 30, 2022
Last update date Aug 25, 2023
Contact name Jason Gertz
E-mail(s) jay.gertz@hci.utah.edu
Organization name University of Utah
Lab Gertz
Street address 1950 Cir of Hope Dr
City Salt Lake City
State/province UT
ZIP/Postal code 84112
Country USA
 
Platforms (1)
GPL32712 NanoString nCounter human v3 miRNA panel
Samples (25)
GSM6612226 T47D_WT_clone1_miRNA_expression
GSM6612227 T47D_WT_clone2_miRNA_expression
GSM6612228 T47D_Y537S_clone1_miRNA_expression
This SubSeries is part of SuperSeries:
GSE214580 Estrogen receptor alpha mutations regulate gene expression and cell growth in breast cancer through microRNAs
Relations
BioProject PRJNA885892

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE214578_RAW.tar 210.0 Kb (http)(custom) TAR (of RCC)
Processed data included within Sample table
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