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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jun 12, 2023 |
| Title |
MOF-mediated histone H4 lysine 16 acetylation governs mitochondrial and ciliary functions by controlling promoter interactions (scATAC-Seq) |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Histone H4 lysine 16 acetylation (H4K16Ac), governed by the histone acetyltransferase (HAT) MOF, orchestrates critical functions in gene expression regulation and chromatin interaction. In this study, we show that conditional genetic deletion of Mof but not Kansl1, the essential component of the NSL complex, causes severe defects during murine skin development. Single-cell and bulk RNA-seq, in combination with MOF ChIP-seq, reveal that numerous MOF targeted and downregulated genes are highly enriched in mitochondria and cilia. Genetic deletion of Uqcrq, an essential subunit for electron transport chain Complex III, recapitulates the defects observed in MOF cKO. Single-cell ATAC-seq reveals that MOF targeted genes are controlled prominently through promoter interactions.
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| Overall design |
scATAC-Seq
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| Web link |
https://www.nature.com/articles/s41467-023-40108-0
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| Contributor(s) |
Wang D, Li H, Chandel N, Dou Y, Yi R |
| Citation(s) |
37479688 |
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| Submission date |
Sep 29, 2022 |
| Last update date |
Sep 11, 2023 |
| Contact name |
Rui Yi |
| E-mail(s) |
yir@northwestern.edu
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| Organization name |
Northwestern University
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| Street address |
303 E. Chicago Avenue
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| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE214441 |
MOF-mediated histone H4 lysine 16 acetylation governs mitochondrial and ciliary functions by controlling promoter interactions |
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| Relations |
| BioProject |
PRJNA885419 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE214439_RAW.tar |
498.3 Mb |
(http)(custom) |
TAR (of BED, MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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