|
| Status |
Public on Aug 01, 2010 |
| Title |
Expression profile of proliferating and differentiating primary human keratinocytes in vitro |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Human epidermal keratinocytes undergo tightly controlled program of cell differentiation, leading to the formation of cornified envelope. Primary keratinocytes in vitro, under calcium stimulation mimic the differentiation program observed in vivo. Analysis of the transcription profile of two cell population, such as proliferating cells and differentiating cells helps to discover new genes implicated in that process and to understand the mechanisms of regulation of the keratinocyte differentiation.
|
| |
|
| Overall design |
Primary human keratinocytes were cultured under proliferating (Day 0, sub-confluent cells) and differentiating (seven days of high calcium medium) conditions. As a source of cells, we used normal skin from different body sites: back, foreskin, palmoplantar. RNA extracted from cultured primary human keratinocytes were isolated from five different donors. We compared the expression profiles of proliferating versus differentiating keratinocytes.
|
| |
|
| Contributor(s) |
Obarzanek-Fojt M, Favre B, Huber M, Hohl D, Harshmann K, Pradervand S, Hagenbüchle O, Paillusson A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 20, 2010 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Magdalena Obarzanek-Fojt |
| E-mail(s) |
magdalena.obarzanek@chuv.ch
|
| Phone |
(41) 21 314 03 64
|
| Fax |
(41) 21 314 03 78
|
| Organization name |
CHUV
|
| Department |
Dermatology
|
| Lab |
Cutanous Biology
|
| Street address |
Beaumont 29
|
| City |
Lausanne |
| ZIP/Postal code |
1011 |
| Country |
Switzerland |
| |
|
| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
|
| Samples (10)
|
|
| Relations |
| BioProject |
PRJNA126355 |
Less...
More...