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| Status |
Public on Jan 01, 2024 |
| Title |
Krüppel-like factor 2 in VCAM1+ endothelial cells is a critical regulator of vascular inflammation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Endothelial cells (ECs) act as an internal barrier to the vessel wall, hindering the development of inflammation, and only when activated will promote the development of vascular inflammation. However, the heterogeneity of the aortic ECs in the setting of vascular inflammation have not been fully assessed. Here, we defined 4 EC subpopulations, including Cd36+ EC, Notch3+ EC, Vcam1+ EC and Lyve1+ EC. Based on the enrichment analysis, Vcam1+ EC were considered to be a subpopulation of ECs susceptible to activation. After high-fat diet (HFD) intake, decreased expression of Krüppel-like factor 2 (Klf2) occurred only in Vcam1+ EC. Moreover, S1pr1 was validated as a downstream target protein of klf2. Upregulation of Klf2 expression ameliorated vascular ECs inflammation under HFD condition. Our study elucidates the Klf2 in Vcam1+ EC is a critical regulator of vascular inflammation and increases understanding of development and progression of aortic inflammatory disease.
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| Overall design |
Single cell RNA-seq on aortic cells of normal diet or high-fat diet mice
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| Contributor(s) |
Kan H, Zhang K, Mao A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Sep 23, 2022 |
| Last update date |
Jan 01, 2024 |
| Contact name |
Hao Kan |
| E-mail(s) |
mars.kan@outlook.com
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| Phone |
+8617714542580
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| Organization name |
jiangnan university
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| Street address |
The number of 1800 ,Li Hu Road
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| City |
Wuxi |
| State/province |
- |
| ZIP/Postal code |
214122 |
| Country |
China |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA883634 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE214014_RAW.tar |
332.4 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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