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Series GSE2140 Query DataSets for GSE2140
Status Public on Aug 01, 2007
Title sigma32 over-expression in E. coli
Platform organism Escherichia coli K-12
Sample organism Escherichia coli
Experiment type Expression profiling by array
Summary This study examined the genes under the control of sigma32 in E. coli by moderate induction of a plasmid-borne rpoH gene under defined steady-state growth condition. Samples were taken from culture at mid log phase (OD=0.2) before or 5 minutes, 10 minutes or 15 minutes after induction. Samples were then RNA-stabilized using Qiagen RNAProtect Bacterial Reagent (Qiagen). Total RNA was then isolated using MasterPure kits (Epicentre Technologies). Purified RNA was reverse-transcribed to cDNA, labeled and hybridized to Affymetrix GeneChip E. coli Antisense Genome Arrays as recommended in the technical manual ( Meanwhile, we measured the protein level of sigma32 during the time course. The changes of some sigma32 –dependent genes were also determined in a DnaK null mutant to examine the anti- sigma32 function of DnaK.
Keywords = sigma factor
Keywords = regulon
Keywords = microarray
Keywords = Western blot
Keywords = E. coli
Keywords = anti-sigma factor
Keywords: time-course
Contributor(s) Zhao K, Liu M, Burgess RR
Citation(s) 15757896
Submission date Jan 10, 2005
Last update date Mar 16, 2012
Contact name Kai Zhao
Phone 608-263-3375
Fax 608-262-2824
Organization name Mcardle Laboratory for Cancer Research
Department Oncology
Lab Richard R. Burgess
Street address 2100 University Avenue
City Madison
State/province WI
ZIP/Postal code 53705
Country USA
Platforms (1)
GPL199 [Ecoli_ASv2] Affymetrix E. coli Antisense Genome Array
Samples (8)
GSM38696 control
GSM38697 5min
GSM38698 10min
BioProject PRJNA91269

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