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Series GSE2140 Query DataSets for GSE2140
Status Public on Aug 01, 2007
Title sigma32 over-expression in E. coli
Platform organism Escherichia coli K-12
Sample organism Escherichia coli
Experiment type Expression profiling by array
Summary This study examined the genes under the control of sigma32 in E. coli by moderate induction of a plasmid-borne rpoH gene under defined steady-state growth condition. Samples were taken from culture at mid log phase (OD=0.2) before or 5 minutes, 10 minutes or 15 minutes after induction. Samples were then RNA-stabilized using Qiagen RNAProtect Bacterial Reagent (Qiagen). Total RNA was then isolated using MasterPure kits (Epicentre Technologies). Purified RNA was reverse-transcribed to cDNA, labeled and hybridized to Affymetrix GeneChip E. coli Antisense Genome Arrays as recommended in the technical manual (www.affymetrix.com). Meanwhile, we measured the protein level of sigma32 during the time course. The changes of some sigma32 –dependent genes were also determined in a DnaK null mutant to examine the anti- sigma32 function of DnaK.
Keywords = sigma factor
Keywords = regulon
Keywords = microarray
Keywords = Western blot
Keywords = E. coli
Keywords = anti-sigma factor
Keywords: time-course
 
 
Contributor(s) Zhao K, Liu M, Burgess RR
Citation(s) 15757896
Submission date Jan 10, 2005
Last update date Mar 16, 2012
Contact name Kai Zhao
E-mail(s) zhao@oncology.wis.edu
Phone 608-263-3375
Fax 608-262-2824
URL http://mcardle.oncology.wisc.edu/burgess
Organization name Mcardle Laboratory for Cancer Research
Department Oncology
Lab Richard R. Burgess
Street address 2100 University Avenue
City Madison
State/province WI
ZIP/Postal code 53705
Country USA
 
Platforms (1)
GPL199 [Ecoli_ASv2] Affymetrix E. coli Antisense Genome Array
Samples (8)
GSM38696 control
GSM38697 5min
GSM38698 10min
Relations
BioProject PRJNA91269

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