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| Status |
Public on Jan 31, 2024 |
| Title |
Xrp1 governs the stress response program to spliceosome dysfunction |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We investigated the changes in gene expression in response to reduced levels of Ecdysoneless (Ecd), and the role of Xrp1 in induction of transcriptional changes upon loss of Ecd in the developing wing disc of Drosophila. To do this we utilized the GAL4/UAS system to express UAS-ecd-RNAi alone and in combination with UAS-xrp1-RNAi in the nubbin-domain (nub-Gal4, UAS-mRFP, UAS-ecd-RNAi and nub-Gal4, UAS-mRFP, UAS-ecd-RNAi, xrp1-RNAi) of the wing disc and used nub-Gal4, UAS-mRFP wing discs as controls. We quantified transcriptomic expression analysis from four biological replicates for both experimental groups
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| Overall design |
mRNA profiles were generated by single-end, total RNA Illumina sequencing (HiSeq2500) from wandering L3 stage wing discs of nub-Gal4, UAS-mRFP, UAS-ecd-RNAi; nub-Gal4, UAS-mRFP, UAS-ecd-RNAi, xrp1-RNAi and nub-Gal4, UAS-mRFP Drosophila larvae.
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| Web link |
https://doi.org/10.1093/nar/gkae055
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| Contributor(s) |
Stankovic D, Tain L, Uhlirova M |
| Citation(s) |
38303573 |
| |
| Submission date |
Sep 13, 2022 |
| Last update date |
Apr 02, 2024 |
| Contact name |
Mirka Uhlirova |
| E-mail(s) |
mirka.uhlirova@uni-koeln.de
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| Organization name |
CECAD Research Center Cologne
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| Department |
Institute for Genetics
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| Lab |
Uhlirova Lab
|
| Street address |
Joseph-Stelzmann Str. 26
|
| City |
Cologne |
| State/province |
NRW |
| ZIP/Postal code |
50931 |
| Country |
Germany |
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| Platforms (1) |
| GPL13304 |
Illumina HiSeq 2000 (Drosophila melanogaster) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA880351 |
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