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Series GSE21278 Query DataSets for GSE21278
Status Public on May 04, 2010
Title A genomic atlas of mouse hypothalamic development
Organism Mus musculus
Experiment type Expression profiling by array
Summary The hypothalamus is a central regulator of many behaviors essential for survival such as temperature regulation, food intake and circadian rhythms. However, the molecular pathways that mediate hypothalamic development are largely unknown. To identify genes expressed in developing mouse hypothalamus, microarray analysis at 12 different developmental time points was performed. Developmental in situ hybridization was conducted for 1,045 genes dynamically expressed by microarray analysis. In this way, we identified markers that stably labeled each major hypothalamic nucleus over the entire course of neurogenesis, and thus constructed a detailed molecular atlas of the developing hypothalamus. As proof of concept for the utility of this data, we used these markers to analyze the phenotype of mice where Sonic Hedgehog (Shh) was selectively deleted from hypothalamic neuroepithelium, demonstrating an essential role for Shh in anterior hypothalamic patterning. Our results serve as a resource for functional investigations of hypothalamic development, connectivity, physiology, and dysfunction.
Overall design Affymetrix MOE430 microarrays were used to analyze the expression patterns of mouse hypothalamic and preoptic area tissues. The results were compared across the variables of Strain, Sex and Age.
Contributor(s) Shimogori T, Lee DA, Miranda-Angulo A, Yang Y, Wang H, Jiang L, Yoshida AC, Mashiko H, Kataoka A, Avetisyan M, Qi L, Qian J, Blackshaw S
Citation(s) 20436479
Submission date Apr 09, 2010
Last update date Feb 11, 2019
Contact name Seth Blackshaw
Organization name The Johns Hopkins University
Department Neuroscience
Street address 733 N. Broadway, Suite 351
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (48)
GSM531810 C57Bl/6 E10 Female, hypothalamus and preoptic area
GSM531818 C57Bl/6 E10 Male, hypothalamus and preoptic area
GSM531831 C57Bl/6 E11 Female, hypothalamus and preoptic area
BioProject PRJNA126583

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Supplementary file Size Download File type/resource
GSE21278_RAW.tar 312.5 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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