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Series GSE21272 Query DataSets for GSE21272
Status Public on Oct 21, 2010
Title Time Course Gene Expression Microarray Analysis of a Mouse B-cell Line (CH12.LX) Activated with Lipopolysaccharide and Treated with 2,3,7,8-Tetrachlorodibenzo-p-dioxin
Organism Mus musculus
Experiment type Expression profiling by array
Summary The objective of the study was to characterize gene expression cascade involved in the suppression of B-cell activation and differentiation by 2,3,7,8-tetrachlorodibenzo-p-dixoxin (TCDD). The underlying hypothesis was that multiple nodes in the B-cell differentiation network are directly or indirectly regulated by TCDD through its receptor, the AHR.
 
Overall design The mouse B-cell line (CH12.LX) was plated at 1X10^5 cells/ml at time 0 and activated with lipopolysaccharide (LPS, Salmonella typhosa). The cells were then treated with either dimethyl sulfoxide (DMSO, 0.01%) or 10 nM 2,3,7,8-tetrachlorodibenzo-p-dixoxin (TCDD). The cells were harvested at 0, 8, 12, 24, 36, and 48 hrs post-treatment. At the 0 hr time point, cells were untreated. A total of 4 experimental replicates per time point per treatment group were included with the cells for each replicate treated and harvested on separate days. Single color Affymetrix Mouse 430 2.0 arrays were used.
 
Contributor(s) Thomas RS, De Abrew N
Citation(s) 20819909
Submission date Apr 08, 2010
Last update date Feb 11, 2019
Contact name Russell Scott Thomas
E-mail rthomas@thehamner.org
Phone 919-558-1311
Fax 919-558-1300
Organization name The Hamner Institutes for Health Sciences
Street address 6 Davis Drive
City Research Triangle Park
State/province NC
ZIP/Postal code 27709
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (44)
GSM501399 LX_ND_0_0_12_CH12LX_UN_2_120408_Mouse430_2_RSTMSU601_9
GSM501400 LX_ND_0_0_1_CH12LX_UN_1_111908_Mouse430_2_RSTMSU601_9
GSM501401 LX_ND_0_0_23_CH12LX_UN_3_121508_Mouse430_2_RSTMSU601_9
Relations
BioProject PRJNA126261

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE21272_RAW.tar 180.4 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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