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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jul 20, 2023 |
| Title |
Role of Gdf15 in neonatal hyperoxic lung injury |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Growth Differentiation Factor 15 (GDF15) is a divergent member of the TGF-β superfamily, and its expression increases under various stress conditions, including inflammation, hyperoxia, and senescence. GDF15 expression is increased in neonatal murine BPD models, and GDF15 loss exacerbates oxidative stress and decreases viability in vitro in pulmonary epithelial and endothelial cells. Our overall hypothesis is that the loss of GDF15 will exacerbate hyperoxic lung injury in the neonatal lung in vivo. We exposed neonatal Gdf15-/- mice and wild-type (WT) controls on a similar background to room air or hyperoxia (95% O2) for 5 days after birth. The mice were euthanized on PND 21. Gdf15 -/- mice had higher mortality and lower body weight than WT mice after exposure to hyperoxia. Upon exposure to hyperoxia, female mice had higher alveolar simplification in the Gdf15-/- group than the female WT group. Gdf15-/- and WT mice showed no difference in the degree of the arrest in angiogenesis upon exposure to hyperoxia. Gdf15-/- mice showed lower macrophage count in the lungs compared to WT mice. Our results suggest that Gdf15 deficiency decreases the tolerance to hyperoxic lung injury with evidence of sex-specific differences.
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| Overall design |
We exposed neonatal Gdf15-/- mice and wild-type (WT) controls both C57BL6 background on a similar background to room air or hyperoxia (95% O2) for 5 days after birth. The mice were euthanized on PND 21.
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| Contributor(s) |
Lingappan K |
| Citation(s) |
37368978 |
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| Submission date |
Aug 21, 2022 |
| Last update date |
Oct 19, 2023 |
| Contact name |
Abiud Cantu |
| E-mail(s) |
cantua@chop.edu
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| Organization name |
Children's Hospital of Philadelphia
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| Department |
Neonatology
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| Lab |
Lingappan Lab
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| Street address |
3615 Civic Center Blvd
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| City |
Philadelphia |
| State/province |
Pennsylvania |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (32)
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| GSM6500488 |
KL-56, KO, Male, Oxygen |
| GSM6500489 |
KL-2, KO, Female, Oxygen |
| GSM6500490 |
KL-4, KO, Female, Oxygen |
| GSM6500491 |
KL-53, KO, Female, Oxygen |
| GSM6500492 |
KL-54, KO, Female, Oxygen |
| GSM6500493 |
KL-16, KO, Male, RoomAir |
| GSM6500494 |
KL-17, KO, Male, RoomAir |
| GSM6500495 |
KL-57, KO, Male, RoomAir |
| GSM6500496 |
KL-58, KO, Male, RoomAir |
| GSM6500497 |
KL-18, KO, Female, RoomAir |
| GSM6500498 |
KL-19, KO, Female, RoomAir |
| GSM6500499 |
KL-61, KO, Female, RoomAir |
| GSM6500500 |
KL-62, KO, Female, RoomAir |
| GSM6500501 |
KL-34, WT, Male, Oxygen |
| GSM6500502 |
KL-35, WT, Male, Oxygen |
| GSM6500503 |
KL-39, WT, Male, Oxygen |
| GSM6500504 |
KL-51, WT, Male, Oxygen |
| GSM6500505 |
KL-36, WT, Female, Oxygen |
| GSM6500506 |
KL-37, WT, Female, Oxygen |
| GSM6500507 |
KL-38, WT, Female, Oxygen |
| GSM6500508 |
KL-52, WT, Female, Oxygen |
| GSM6500509 |
KL-28, WT, Male, RoomAir |
| GSM6500510 |
KL-29, WT, Male, RoomAir |
| GSM6500511 |
KL-41, WT, Male, RoomAir |
| GSM6500512 |
KL-42, WT, Male, RoomAir |
| GSM6500513 |
KL-30, WT, Female, RoomAir |
| GSM6500514 |
KL-31, WT, Female, RoomAir |
| GSM6500515 |
KL-47, WT, Female, RoomAir |
| GSM6500516 |
KL-48, WT, Female, RoomAir |
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| Relations |
| BioProject |
PRJNA871940 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE211744_RAW.tar |
59.2 Mb |
(http)(custom) |
TAR (of TAR) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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