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Series GSE21133 Query DataSets for GSE21133
Status Public on Apr 09, 2010
Title Identification of Components Associated with Thermal Acclimation of Photosystem II in Synechocystis sp. PCC6803
Organism Synechocystis sp. PCC 6803
Experiment type Expression profiling by array
Summary Photosystem II (PSII) is the most thermally sensitive component of photosynthesis. Thermal acclimation of this complex activity is likely to be critically important to the ability of photosynthetic organisms to tolerate temperature changes in the environment.
We have analysed gene expression using whole-genome microarrays and monitored alterations in physiology during acclimation of PSII to elevated growth temperature in Synechocystis sp. PCC 6803. PSII acclimation is complete within 480 minutes of exposure to elevated temperature and is associated with a highly dynamic transcriptional response. 176 genes were identified and classified into seven distinct response profile groups. Response profiles suggest the existence of an early transient phase and a sustained phase to the acclimation response. The early phase was characterised by induction of general stress response genes, including heat shock proteins, which are likely to influence PSII thermal stability. The sustained phase consisted of acclimation-specific alterations that are involved in other cellular processes. Sustained responses included genes involved in phycobillisome structure and modification, photosynthesis, respiration, lipid metabolism and motility. Approximately 60% of genes with sustained altered expression levels have no known function. The potential role of differentially expressed genes in thermotolerance and acclimation is discussed. We have characterised the acclimation physiology of selected gene ‘knockouts’ to elucidate possible gene function in the response.
All mutants show lower PSII rates under normal growth conditions. Basal PSII thermotolerance was affected by mutations in clpB1, cpcC2, hspA, htpG and slr1674. Final PSII thermotolerance was affected by mutations in cpcC2, hik34, hspA and hypA1, suggesting that these gene products play roles in long-term thermal acclimation of PSII.
Overall design Gene expression levels were compared during a time-course (up to 8 hours) of thermal acclimation (from 25C to 38C) of a wild-type strain grown under continuous illumination and bubbled with CO2 -enriched air. Up to four independent biological replicates were generated and analysed. Each array: replicate matched time-point vs. 0hr control.
Contributor(s) Rowland JG, Pang X, Suzuki I, Murata N, Simon WJ, Slabas AR
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Submission date Mar 30, 2010
Last update date Mar 22, 2012
Contact name John G Rowland
Organization name Durham University
Department Biological & Biomedical Sciences
Street address South Road
City Durham
ZIP/Postal code DH1 3LE
Country United Kingdom
Platforms (1)
GPL4017 TakaraBio CyanoCHIP ver1.4-ver1.6
Samples (22)
GSM528566 480 minute acclimation response - replicate 1
GSM528567 480 minute acclimation response - replicate 2
GSM528568 480 minute acclimation response - replicate 3
BioProject PRJNA126647

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Supplementary file Size Download File type/resource
GSE21133_RAW.tar 30.2 Mb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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