|
| Status |
Public on Apr 21, 2023 |
| Title |
scRNA-seq of Gene-edited Porcine Kidneys Prior to Xenotransplantation into Cynomolgus Macaques |
| Organism |
Sus scrofa |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Succesful xenotransplantation of porcine kidneys into humans requires the ubiqutous expression of certain human transgenes. Modern transcriptomics now alllows gene expression to be measured at the per-cell level, which can provide important insights into the expression pattern of these life-sustaining transgenes in a complex organ such as the kidney. Analysis of this scRNA-seq data shows ubiquitous expression of transgenes in most major kidney cell populations which includes various subtypes of endothelial cells.
|
| |
|
| Overall design |
10X 3' scRNA-seq was performed on gene-edited porcine contralateral kidneys prior to xenotransplantation into cynomolgus macaques to measure transgene expression at cellular resolution.
|
| |
|
| Contributor(s) |
Chhangawala S, Policastro RA |
| Citation(s) |
37311769, 37821590 |
| |
| Submission date |
Aug 04, 2022 |
| Last update date |
Oct 26, 2023 |
| Contact name |
Robert Policastro |
| Organization name |
Ensoma Inc.
|
| Street address |
451 D St Unit #600
|
| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02210 |
| Country |
USA |
| |
|
| Platforms (1) |
|
| Samples (4)
|
|
| This SubSeries is part of SuperSeries: |
| GSE211432 |
Life-supporting function of genome edited porcine renal graft in cynomolgus macaques |
|
| Relations |
| BioProject |
PRJNA866159 |
Less...
More...