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Series GSE21037 Query DataSets for GSE21037
Status Public on Nov 11, 2010
Title L1 retrotransposition in neurons is mediated by MeCP2
Organism Homo sapiens
Experiment type Expression profiling by array
Summary L1 retrotransposons are active elements in the genome, capable of mobilization in neuronal progenitor cells. Previously, we showed that chromatin remodeling during neuronal differentiation allows for a transient stimulation of L1 transcription. The activity of L1 retrotransposons during brain development can impact gene expression and neuronal function. Here we show that L1 neuronal retrotransposition in rodents is increased in the absence of MeCP2, a protein involved in global methylation and human neurodevelopmental diseases. Using neuronal progenitor cells derived from human induced pluripotent stem cells and human tissues, we revealed that Rett syndrome patients, with MeCP2 mutations, have increased susceptibility for L1 retrotransposition. Our data demonstrate that disease-related genetic mutations can influence the frequency of neuronal L1 retrotransposition, thereby increasing brain-specific genetic mosaicism.
Overall design Genetic reprogramming of somatic cells to a pluripotent state (induced pluripotent stem cells, or iPSCs) by over-expression of specific genes has been accomplished for fibroblasts derived from controls and Rett syndrome patients. Different clones from each were compared to respective original fibroblasts and a human embryonic stem cell line.
Gene expression profiles measured using human genome Affymetrix Gene Chip arrays were grouped by hierarchical clustering, and correlation coefficients were computed for all pair-wise comparisons.
Contributor(s) Muotri A, Marchetto M, Yeo G, Gage F
Citation(s) 21074045
Submission date Mar 23, 2010
Last update date Jul 26, 2018
Contact name Alysson R Muotri
Phone 858-534-9320
Fax 858-534-9320
Organization name University of California San Diego
Department Pediatrics/CMM
Street address 9500 Gilman Drive
City La Jolla
State/province CA
ZIP/Postal code 92093-0695
Country USA
Platforms (1)
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
Samples (21)
GSM525406 RTT fibroblast, rep1
GSM525407 RTT fibroblast, rep2
GSM525408 RTT fibroblast, rep3
BioProject PRJNA126459

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Supplementary file Size Download File type/resource
GSE21037_RAW.tar 80.2 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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