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Series GSE20986 Query DataSets for GSE20986
Status Public on Dec 31, 2010
Title Comparative Gene Expression Profiling of HUVEC and Ocular Vascular Endothelial Cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To compare the gene expression profiles of unpassaged, proliferating HUVEC and human iris, retinal and choroidal microvascular endothelial cells.
Gene expression profiling revealed significant differences between HUVEC and ocular microvascular endothelial cells suggesting that HUVE cells may not be a suitable surrogate when studying pathophysiological mechanisms of ocular disorders. There were significant differences in the gene expression of important cell signalling pathways in human retinal and choroidal ECs. These differences may be important in the mechanisms and treatment of choroidal and retinal neovascularisation.
Overall design 12 arrays are included. Endothelial cells were derived from 4 tissues: iris, retina, choroid and human umbilical vein. RNA extracts from cells were hybridised to Affymetrix HGU133plus2 arrays in triplicate.
Contributor(s) Browning AC, Swan DC
Citation(s) 22028475
Submission date Mar 20, 2010
Last update date Mar 25, 2019
Contact name Simon Cockell
Organization name Newcastle University
Street address Framlington Place
City Newcastle
ZIP/Postal code NE2 4HH
Country United Kingdom
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (12)
GSM524662 iris-cellline-1
GSM524663 retina-cellline-1
GSM524664 retina-cellline-2
BioProject PRJNA124497

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE20986_RAW.tar 53.8 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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