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| Status |
Public on Jan 11, 2023 |
| Title |
Transcriptome analysis of the binucleate ciliate Tetrahymena thermophila with asynchronous nuclear cell cycles |
| Organism |
Tetrahymena thermophila |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
As a prototypic ciliated protozoan, Tetrahymena thermophila harbors two functionally and physically distinct nuclei within a shared cytoplasm. During the vegetative phase of the life cycle, the S phases of the diploid germline micronucleus and polyploid somatic macronucleus are temporally offset. Here we report the first RNA-Seq analysis across the cell cycle of a binucleated ciliated protozoan.
We then performed gene expression profiling analysis using data obtained from RNA-seq of 4 different cells at two time points.
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| Overall design |
RNA was isolated at 30 min intervals across 1.5 vegetative cell cycles, starting with a G1 population synchronized by centrifugal elutriation.
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| Contributor(s) |
Zhang L, Cervantes M, Pan S |
| Citation(s) |
36475712 |
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| Submission date |
Jul 24, 2022 |
| Last update date |
Jul 17, 2024 |
| Contact name |
GEOFFREY KAPLER |
| E-mail(s) |
gkapler@tamu.edu
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| Organization name |
Texas A&M University
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| Street address |
4209B Medical Research Education Building II
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| City |
Bryan |
| State/province |
TX |
| ZIP/Postal code |
77807 |
| Country |
USA |
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| Platforms (1) |
| GPL23968 |
Illumina HiSeq 2500 (Tetrahymena thermophila) |
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| Samples (18)
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| GSM6380746 |
Vegetative growing cells, 0 min after elutriation, A |
| GSM6380747 |
Vegetative growing cells, 0 min after elutriation, B |
| GSM6380748 |
Vegetative growing cells, 30 min after elutriation, A |
| GSM6380749 |
Vegetative growing cells, 30 min after elutriation, B |
| GSM6380750 |
Vegetative growing cells, 60 min after elutriation, A |
| GSM6380751 |
Vegetative growing cells, 60 min after elutriation, B |
| GSM6380752 |
Vegetative growing cells, 90 min after elutriation, A |
| GSM6380753 |
Vegetative growing cells, 90 min after elutriation, B |
| GSM6380754 |
Vegetative growing cells, 120 min after elutriation, A |
| GSM6380755 |
Vegetative growing cells, 120 min after elutriation, B |
| GSM6380756 |
Vegetative growing cells, 150 min after elutriation, A |
| GSM6380757 |
Vegetative growing cells, 150 min after elutriation, B |
| GSM6380758 |
Vegetative growing cells, 180 min after elutriation, A |
| GSM6380759 |
Vegetative growing cells, 180 min after elutriation, B |
| GSM6380760 |
Vegetative growing cells, 210 min after elutriation, A |
| GSM6380761 |
Vegetative growing cells, 210 min after elutriation, B |
| GSM6380762 |
Vegetative growing cells, 240 min after elutriation, A |
| GSM6380763 |
Vegetative growing cells, 240 min after elutriation, B |
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| Relations |
| BioProject |
PRJNA861835 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE209619_gene_count_cellCycle.csv.gz |
813.5 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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