|
| Status |
Public on Feb 26, 2024 |
| Title |
RNA-seq for blood Ly6Chi and Ly6Clo monocyte in WT and RBP-Jfl/fl Lyz2-Cre mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To evaluate the consequences of RBP-J loss of function in blood monocyte subsets, we sequenced RNA of blood Ly6Chi monocyte and Ly6Clo monocyte from WT and RBP-Jfl/fl Lyz2-Cre mice.
|
| |
|
| Overall design |
Blood Ly6Chi and Ly6Clo monocytes were isolated from WT and RBP-Jfl/fl Lyz2-Cre mice, and total RNA was extracted using total RNA purification kit. RNA was converted into RNA-seq quantification libraries, and RNA-seq libraries were sequenced with the pair-end option.
|
| |
|
| Contributor(s) |
Kang L, Zhang B, Kou T |
| Citation(s) |
38407952 |
| |
| Submission date |
Jul 21, 2022 |
| Last update date |
Mar 20, 2024 |
| Contact name |
Tiantian Kou |
| Organization name |
Tsinghua university
|
| Street address |
Haidian District
|
| City |
Beijing |
| ZIP/Postal code |
100084 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (4)
|
| GSM6369153 |
RBP-Jfl/fl Lyz2-Cre blood Ly6Chi monocyte |
|
| Relations |
| BioProject |
PRJNA860977 |