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Series GSE20870 Query DataSets for GSE20870
Status Public on May 28, 2010
Title The telomere-binding protein Tbf1 demarcates snoRNA gene promoters in Saccharomyces cerevisiae
Organism Saccharomyces cerevisiae
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The majority of Saccharomyces cerevisiae snoRNA promoters contain an aRCCCTaa sequence motif located at the upstream border of a TATA-containing nucleosome-free region. Genome-wide ChIP-seq analysis showed that these motifs are bound in vivo by Tbf1, a telomere-binding protein known to recognize mammalian-like T2AG3 repeats at sub-telomeric regions. Tbf1 has over 100 additional promoter targets, including the TBF1 gene itself. Tbf1 is required for full snoRNA expression, yet it does not influence nucleosome positioning at snoRNA promoters.
 
Overall design Analysis of Tbf1-binding sites in Saccharomyces cerevisiae by ChIP-seq of a Myc-tagged strain and a control untagged strain. 1 sample per strain, 1 lane per sample.
 
Contributor(s) Rougemont J, Naef F, Shore D, Dieci G, Ribeyre C
Citation(s) 20513435
Submission date Mar 12, 2010
Last update date May 15, 2019
Contact name Jacques Rougemont
E-mail(s) jacques.rougemont@epfl.ch
Organization name EPFL
Department School of Life Sciences
Lab Bioinformatics and Biostatistics Core Facility
Street address Station 15
City Lausanne
ZIP/Postal code 1015
Country Switzerland
 
Platforms (1)
GPL9134 Illumina Genome Analyzer (Saccharomyces cerevisiae)
Samples (2)
GSM521934 Yeast_Untagged_control
GSM521935 Yeast_Tbf1_Chip_seq
Relations
SRA SRP002526
BioProject PRJNA124661

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE20870_RAW.tar 101.3 Mb (http)(custom) TAR (of BED, BEDGRAPH, MAP)
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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