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| Status |
Public on Feb 06, 2023 |
| Title |
Gene expression profile at single cell level of hematopoietic progenitors of the bone marrow and spleen with or without the presence PyMT-B6 tumor |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Hematopoietic stem and progenitors cells interact with organ specific stem cell niches. We used single cell RNA sequencing (scRNA-seq) to analyze the gene expression of hematopoietic and potential stromal cells in the bone marrow and spleen with or without PyMT-B6 breast cancer.
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| Overall design |
Bone marrow and splenic hematopoietic stem and progenitor cells as well as a variety of stromal cell populations were isolated from mice with or without PyMT-B6 tumors using fluorescence-activated cell sorting (FACS).
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| Contributor(s) |
Barisas D, Choi K |
| Citation(s) |
37134077 |
| |
| Submission date |
Jul 11, 2022 |
| Last update date |
Aug 02, 2023 |
| Contact name |
Derek Arthur George Barisas |
| Organization name |
Washington University in Saint Louis
|
| Department |
Pathology and Immunology
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| Lab |
Kyunghee Choi
|
| Street address |
660 South Euclid
|
| City |
Saint Louis |
| State/province |
Missouri |
| ZIP/Postal code |
63110 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA857713 |
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