|
| Status |
Public on Jun 26, 2024 |
| Title |
Chromatin reorganization drives expression of functional maturation genes in senescent pancreatic beta cells (Series1 - Transcriptome) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Using a unique model of cultured human beta cell senescence we show that chromatin reorganization leads to activation of enhancers regulating functional maturation genes, concomitantly with acquisition of glucose-stimulated insulin secretion capacity. Interferon-response genes are elevated in senescent beta cells, but cytokine-encoding senescence-associated secretory phenotype (SASP) genes are not. Human beta cell senescence thus involves chromatin-driven upregulation of a functional maturation program and of interferon-stimulated genes, changes that could increase both insulin secretion and immune reactivity.
|
| |
|
| Overall design |
Expression profiles of non-senescent and senescent human pancreatic beta-like cells in culture.
|
| |
|
| Contributor(s) |
Ben-Porath I, Dor Y, Patra M |
| Citation(s) |
38682582 |
| |
| Submission date |
Jul 09, 2022 |
| Last update date |
Jun 26, 2024 |
| Contact name |
Milan Patra |
| E-mail(s) |
patra.milan@mail.huji.ac.il
|
| Phone |
+972586913473
|
| Organization name |
The Hebrew University of Jerusalem
|
| Department |
Faculty of Medicine, Department of Developmental Biology and Cancer Research
|
| Street address |
Hadassah Ein Kerem
|
| City |
Jerusalem |
| ZIP/Postal code |
91120 |
| Country |
Israel |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (12)
|
| GSM6319699 |
EndoC-βH3 cells, Non senescent cells A1 |
| GSM6319700 |
EndoC-βH3 cells, Non senescent cells A2 |
| GSM6319701 |
EndoC-βH3 cells, Non senescent cells B1 |
|
| This SubSeries is part of SuperSeries: |
| GSE207826 |
Chromatin reorganization drives expression of functional maturation genes in senescent pancreatic beta cells |
|
| Relations |
| BioProject |
PRJNA857278 |
Less...
More...